HSD17B4 Antibody

Images

 
Western Blot: HSD17B4 Antibody [NBP1-33192] - HSD17B4 antibody detects HSD17B4 protein A. 20 ug Rat heart lysate/extract 7.5% SDS-PAGE HSD17B4 antibody dilution: 1:3000
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody [NBP1-33192] - Analysis of methanol-fixed Hep3B, using HSD17B4antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - Paraffin-embedded rat heart. HSD17B4 antibody diluted at 1:500.
Western Blot: HSD17B4 Antibody [NBP1-33192] - 30 ug of Hep G2 whole cell lysate, 7.5% SDS PAGE, NBP1-33192 diluted at 1:1000.
Western Blot: HSD17B4 Antibody [NBP1-33192] - Mouse liver whole cell lysate (50 ug) probed with HSD17B4 Antibody (NBP1-33192) diluted at 1:1000.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - Paraffin-embedded DLD-1 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - Paraffin-embedded rat testis. HSD17B4 antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HSD17B4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HSD17B4. The exact sequence is proprietary.
Localization
Peroxisome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSD17B4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (87%), Porcine (87%), Bovine (88%), Guinea Porcine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSD17B4 Antibody

  • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
  • 7-alpha
  • D-bifunctional protein
  • EDH17B4
  • hydroxysteroid (17-beta) dehydrogenase 4
  • MPF-2
  • peroxisomal multifunctional enzyme type 2,17-beta-HSD IV
  • peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV
  • peroxisomal
  • short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HSD17B4
Entrez
Uniprot