HSD17B4 Antibody - BSA Free

Western Blot: HSD17B4 Antibody [NBP1-33192] - HSD17B4 antibody detects HSD17B4 protein A. 20 ug Rat heart lysate/extract 7.5% SDS-PAGE HSD17B4 antibody dilution: 1:3000

Immunocytochemistry/Immunofluorescence: HSD17B4 Antibody [NBP1-33192] - Analysis of methanol-fixed Hep3B, using HSD17B4antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - Rat heart. HSD17B4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - DLD-1 xenograft , using HSD17B4 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-33192] - Rat testis. HSD17B4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

3T3-L1 and differentiated 3T3-L1 (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSD17B4 antibody (NBP1-33192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSD17B4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HSD17B4. The exact sequence is proprietary.
• Localization: Peroxisome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSD17B4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31757939).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HSD17B4 Antibody - BSA Free

• 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
• 7-alpha
• D-bifunctional protein
• EDH17B4
• hydroxysteroid (17-beta) dehydrogenase 4
• MPF-2
• peroxisomal multifunctional enzyme type 2,17-beta-HSD IV
• peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV
• peroxisomal
• short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Background

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HSD17B4 Antibody (NBP1-33192)(1)

Publication using NBP1-33192

• DAvalos-Salas M, Montgomery MK, Reehorst CM et al. Deletion of intestinal Hdac3 remodels the lipidome of enterocytes and protects mice from diet-induced obesity Nat Commun 2019-11-22 [PMID: 31757939] (WB, Mouse)

Bioinformatics

• Gene Symbol: HSD17B4
• Entrez:
  • 3295(Human)
  • 15488(Mouse)
• Uniprot:
  • P51659(Human)