![Immunoprecipitation: HRP-2 Antibody [NBP2-32069] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-HRP-2 antibody NBP2-32069 used for IP at 6 ug per reaction. For blotting immunoprecipitated HRP-2, NBP2-32069 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/HRP-2-Antibody-Immunoprecipitation-NBP2-32069-img0001.jpg "Immunoprecipitation: HRP-2 Antibody [NBP2-32069] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-HRP-2 antibody NBP2-32069 used for IP at 6 ug per reaction. For blotting immunoprecipitated HRP-2, NBP2-32069 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit HRP-2 Antibody - BSA Free (NBP2-32069) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 425 to 475 of human Hepatoma-Derived Growth Factor-Related Protein 2 using the numbering given in entry NP_001001520.1 (GeneID 84717). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HDGFL2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is only recommended for use in WB for blotting IP'd samples using the same antibody. Use rabbit anti-HRP-2 antibody NBP2-32074 in traditional WB of non IPd samples (HEK293T, Hela, Jurkat). |
| Storage | Store at 4°C. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for HRP-2 Antibody (NBP2-32069)Find related products by research area.
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| Gene Symbol | HDGFL2 |
| Uniprot |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")