![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Western blot analysis of HPRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 2: Hela cell lysate Lane 2: A431 cell lysate](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0007.jpg "Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Western blot analysis of HPRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 2: Hela cell lysate Lane 2: A431 cell lysate")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, IHC, IP |
| Clone | JU03-26 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit HPRT Antibody (JU03-26) (NBP2-75528) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. Anti-HPRT Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human HPRT aa 169-218 / 218. (SwissProt: P00492 Human; SwissProt: P00493 Mouse; SwissProt: P27605 Rat) |
| Localization | Cytoplasm. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HPRT1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IP-Assay dependent |
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| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HPRT Antibody (NBP2-75528)Find related products by research area.
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| Gene Symbol | HPRT1 |
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0004.jpg "Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.")
![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Hela cell using anti-HPRT antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: A431Lane 3: MCF-7 Lane 4: 293Lane 5: Rat kidney Lane 6: Rat brainLane 7: Mouse testis Lane 8: Mouse colon](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0005.jpg "Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Hela cell using anti-HPRT antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: A431Lane 3: MCF-7 Lane 4: 293Lane 5: Rat kidney Lane 6: Rat brainLane 7: Mouse testis Lane 8: Mouse colon")
![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0006.jpg "Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0001.jpg "Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0002.jpg "Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human kidney tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0003.jpg "Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human kidney tissue using anti-HPRT antibody. Counter stained with hematoxylin.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")