HPRT Antibody (5F11A7) - BSA Free

Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.

Immunohistochemistry-Paraffin: HPRT Antibody (5F11A7) [NBP2-37245] - Analysis of kidney tissues using HPRT1 mouse mAb with DAB staining.

Flow (Intracellular): HPRT Antibody (5F11A7) [NBP2-37245] - An intracellular stain was performed on HeLa cells with HPRT Antibody (5F11A7) NBP2-37245APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mAb against human HPRT1 (AA: FULL(1-218)) recombinant protein. (Expected MW is 50.5 kDa)

Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: HPRT Antibody (5F11A7) [NBP2-37245] - Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).

ELISA: HPRT Antibody (5F11A7) [NBP2-37245] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 5F11A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HPRT Antibody (5F11A7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human HPRT (AA: FULL(1-218)) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HPRT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 24.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HPRT Antibody (5F11A7) - BSA Free

• EC 2.4.2.8
• HGPRT
• HGPRT1
• HGPRTHPRTHGPRTase
• HPRT
• HPRT1
• hypoxanthine phosphoribosyltransferase 1
• hypoxanthine-guanine phosphoribosyltransferase

Background

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HPRT1