| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2518SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52420-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52433-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89672-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52434-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20127-0.025mg | |||
![Western Blot: HP1 alpha Antibody [NB100-2518] - Detection of HP 1 alpha in HeLa whole cell lysate using NB100-2518.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Western-Blot-NB100-2518-img0003.jpg "Western Blot: HP1 alpha Antibody [NB100-2518] - Detection of HP 1 alpha in HeLa whole cell lysate using NB100-2518.")
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody [NB100-2518] - HP1 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2518-img0004.jpg)
![Flow (Intracellular): HP1 alpha Antibody [NB100-2518] - Analysis using the Alexa Fluor 488 conjugate of NB100-2518. Detection of HeLa cells (1 x 10^6 cells/mL) with HP-1 antibody (orange) stained at 1:500. Shown with rIgG (AF488) isotype control (blue).](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Flow-Intracellular-NB100-2518-img0008.jpg)
![Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using HP1 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), and A431 (5) cell lysate.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-2H4E9-Western-Blot-NBP2-52420-img0006.jpg "Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using HP1 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), and A431 (5) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of Hela cells using HP1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-2H4E9-Immunocytochemistry-Immunofluorescence-NBP2-52420-img0007.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of colon tissues using CBX5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-2H4E9-Immunohistochemistry-NBP2-52420-img0003.jpg)
![Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-2H4E9-Western-Blot-NBP2-52420-img0005.jpg)
![Western Blot: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-2H4E9-Western-Blot-NBP2-52420-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Immunofluorescence analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-2H4E9-Immunofluorescence-NBP2-52420-img0001.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Analysis of colon cancer tissues using CBX5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-2H4E9-Immunohistochemistry-NBP2-52420-img0002.jpg)
![ELISA: HP1 alpha Antibody (2H4E9) [NBP2-52420] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-2H4E9-ELISA-NBP2-52420-img0008.jpg)
![Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Western blot analysis of extracts of various cell lines, using HP1 alphaRabbit mAb (NBP3-16267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-8C3M6-Western-Blot-NBP3-16267-img0005.jpg "Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Western blot analysis of extracts of various cell lines, using HP1 alphaRabbit mAb (NBP3-16267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Western blot analysis of extracts of various cell lines, using HP1 alphaRabbit mAb (NBP3-16267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-8C3M6-Western-Blot-NBP3-16267-img0004.jpg)
![Immunoprecipitation: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug HP1 alpha antibody (NBP3-16267). Western blot was performed from the immunoprecipitate using HP1 alpha antibody (NBP3-16267) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-8C3M6-Immunoprecipitation-NBP3-16267-img0006.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-5220251265344.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-52202511411433.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-5220251265335.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-52202511402710.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-522025125446.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-5220251242530.jpg)
![Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Confocal imaging of C6 cells using HP1 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-52202511361212.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-52202511361247.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-52202511365182.jpg)
![Immunohistochemistry: HP1 alpha Antibody (8C3M6) [NBP3-16267] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using HP1 alpha Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16267_rabbit-hp1-alpha-mab-8c3m6-52202511365110.jpg)
![Western Blot: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Western-Blot-NBP2-52433-img0007.jpg "Western Blot: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Immunofluorescence-NBP2-52433-img0002.jpg)
![Immunohistochemistry: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis of paraffin-embedded esophagus tissues using CBX5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Immunohistochemistry-NBP2-52433-img0004.jpg)
![Western Blot: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Western-Blot-NBP2-52433-img0005.jpg)
![Western Blot: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Western-Blot-NBP2-52433-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Immunofluorescence analysis of Hela cells using CBX5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Immunofluorescence-NBP2-52433-img0001.jpg)
![Immunohistochemistry: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Analysis of paraffin-embedded esophageal cancer tissues using CBX5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-Immunohistochemistry-NBP2-52433-img0003.jpg)
![ELISA: HP1 alpha Antibody (1B10B2) [NBP2-52433] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-1B10B2-ELISA-NBP2-52433-img0008.jpg)
![Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Western-Blot-NB110-40623-img0002.jpg "Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).")
![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunohistochemistry-NB110-40623-img0015.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NBP1-89672] - Analysis in human parathyroid gland and skeletal muscle tissues. Corresponding CBX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89672-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HP1 alpha Antibody [NBP1-89672] - Analysis in human parathyroid gland and skeletal muscle tissues. Corresponding CBX5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HP1 alpha Antibody [NBP1-89672] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Western-Blot-NBP1-89672-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody [NBP1-89672] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89672-img0010.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NBP1-89672] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89672-img0015.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NBP1-89672] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89672-img0007.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NBP1-89672] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89672-img0012.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NBP1-89672] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89672-img0013.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NBP1-89672] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-89672-img0014.jpg)
![Western Blot: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-3A11F8-Western-Blot-NBP2-52434-img0006.jpg "Western Blot: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-3A11F8-Immunofluorescence-NBP2-52434-img0003.jpg)
![Flow Cytometry: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Flow cytometric analysis of Hela cells using CBX5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-3A11F8-Flow-Cytometry-NBP2-52434-img0001.jpg)
![Western Blot: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-3A11F8-Western-Blot-NBP2-52434-img0005.jpg)
![Western Blot: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-3A11F8-Western-Blot-NBP2-52434-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Analysis of Hela cells using CBX5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-3A11F8-Immunofluorescence-NBP2-52434-img0002.jpg)
![ELISA: HP1 alpha Antibody (3A11F8) [NBP2-52434] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-3A11F8-ELISA-NBP2-52434-img0007.jpg)
![Western Blot: HP1 alpha Antibody [NBP2-24602] - Analysis of CBX5 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Western-Blot-NBP2-24602-img0001.jpg "Western Blot: HP1 alpha Antibody [NBP2-24602] - Analysis of CBX5 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: HP1 alpha Antibody (RAB-C133) - Chimeric - Azide and BSA Free [NBP3-20127] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C133 (NBP3-20127, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20127_rabbit-hp1-alpha-mab-rab-c133-chimeric-azide-bsa-free-2742023924265.png "Flow Cytometry: HP1 alpha Antibody (RAB-C133) - Chimeric - Azide and BSA Free [NBP3-20127] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C133 (NBP3-20127, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: HP1 alpha Antibody (RAB-C133) - Chimeric - Azide and BSA Free [NBP3-20127] - Nuclear lysates of HeLa(A), HEK293(B), A431(C), MCF7(D), Jurkat(E) and K562(F) cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C133 (NBP3-20127) at 0.003 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20127_rabbit-hp1-alpha-mab-rab-c133-chimeric-azide-bsa-free-2742023924261.png)
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody (RAB-C133) - Chimeric - Azide and BSA Free [NBP3-20127] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C133 (NBP3-20127) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20127, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20127_rabbit-hp1-alpha-mab-rab-c133-chimeric-azide-bsa-free-2742023924264.png)
![Western Blot: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1alpha,beta and gamma diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1alpha, HP1beta and HP1gamma is indicated on the right.](http://images.novusbio.com/fullsize/HP1-Alpha--Beta--and-Gamma-Antibody-Western-Blot-NBP2-59154-img0001.jpg "Western Blot: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1alpha,beta and gamma diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1alpha, HP1beta and HP1gamma is indicated on the right.")
![Chromatin Immunoprecipitation: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - ChIP assays were performed using NIH3T3 cells and 4 ug of the antibody directed against HP1alpha,beta and gamma. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure shows the relative enrichment as compared to a no antibody negative control ChIP.](http://images.novusbio.com/fullsize/HP1-Alpha--Beta--and-Gamma-Antibody-Chromatin-Immunoprecipitation-NBP2-59154-img0002.jpg)
![Immunofluorescence: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - HeLa cells were stained with the antibody against HP1alpha, beta and gamma; and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1alpha,beta and gamma antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HP1-Alpha-Beta-and-Gamma-Antibody-Immunofluorescence-NBP2-59154-img0003.jpg)
![Western Blot: HP1 alpha Antibody [NBP2-59224] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1 alpha, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1 alpha, HP1 beta and HP1 gamma is indicated on the right. The Western blot analysis clearly demonstrates that the HP1 alpha antibody specifically recognizes HP1 alpha and not HP1 beta and HP1 gamma.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Western-Blot-NBP2-59224-img0003.jpg "Western Blot: HP1 alpha Antibody [NBP2-59224] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1 alpha, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1 alpha, HP1 beta and HP1 gamma is indicated on the right. The Western blot analysis clearly demonstrates that the HP1 alpha antibody specifically recognizes HP1 alpha and not HP1 beta and HP1 gamma.")
![Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody [NBP2-59224] - HeLa cells were stained with the antibody against HP1alpha and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1alpha antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Immunofluorescence-NBP2-59224-img0001.jpg)