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Host Cell Factor 1/HCFC1 Antibody

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Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.
Independent Antibodies: Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 ...read more
Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell ...read more

Product Details

Summary
Reactivity Hu, Mu, SyHaSpecies Glossary
Applications WB, Simple Western, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

        

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Host Cell Factor 1/HCFC1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1985 and 2035 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).
Predicted Species
Golden Syrian Hamster (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HCFC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Simple Western
  • Western Blot 1:2000-1:10000
Reviewed Applications
Read 1 Review rated 5
using
NB100-68210 in the following applications:
Publications
Read Publications using
NB100-68210 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Host Cell Factor 1/HCFC1 Antibody

  • C1 Factor
  • CFF
  • CFFC1 factor
  • HCF
  • HCF1
  • HCF1HFC1
  • HCF-1VP16 accessory protein
  • HCFC1
  • HFC1
  • host cell factor C1 (VP16-accessory protein)
  • MGC70925
  • VCAFhost cell factor 1

Background

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-24677
Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-24677] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-OCT1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-24677] - Analysis of OCT-1 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Western Blot: OCT1 Antibody [NBP2-24677] - Western blot analysis of OCT-1 in human HL60 cell lysate using NBP2-24677 at 4 ug/ml.
Species: Ca, Eq, Hu, Pm
Applications: ICC/IF, IHC, IHC-P, WB
OCT2 Antibody
(OCT2/2137)
NBP2-75734
Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Tonsil stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Western Blot: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Analysis Ramos cell lysate using Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Species: Hu
Applications: ELISA, IHC, IHC-P, PA, WB
c-jun [p Ser63]
Antibody (SY0297)
NBP2-67471
Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
CREB3 Antibody
(3H5)
H00010488-M01
Immunohistochemistry-Paraffin: CREB3 Antibody (3H5) [H00010488-M01] - Analysis of monoclonal antibody to CREB3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: CREB3 Antibody (3H5) [H00010488-M01] - Analysis of CREB3 over-expressed 293 cell line, cotransfected with CREB3 Validated Chimera RNAi ( Cat # H00010488-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CREB3 monoclonal antibody (M01), clone 3H5 (Cat # H00010488-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: CREB3 Antibody (3H5) [H00010488-M01] - Analysis of CREB3 expression in transfected 293T cell line by CREB3 monoclonal antibody (M01), clone 3H5.Lane 1: CREB3 transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, S-ELISA, WB
BOB1 Antibody
(BOB1/2424)
NBP2-75752
Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Western Blot: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Analysis of Raji cell lysate using BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Flow Cytometry: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. BOB1 Antibody (BOB1/2424).; followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PA, WB
c-Fos Antibody
(2H2)
NBP2-50037
Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
(1 Review)
(9 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
FosB/G0S3 Antibody
(SN703)
NBP2-67710
Immunohistochemistry-Paraffin: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of paraffin-embedded mouse brain tissue using anti-FosB antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of paraffin-embedded rat spleen tissue using anti-FosB antibody. Counter stained with hematoxylin.
Western Blot: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of FosB on human liver lysates using anti-FosB antibody at 1/1,000 dilution.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
JunD [p Ser255]
Antibody (JJ08-21)
NBP2-67861
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
JunB/AP-1 Antibody
NBP1-89544
Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
SP1 Antibody
NB600-232
Western Blot: SP1 Antibody [NB600-232] - A. Whole cell lysate (50 ug for E; 10 ug for T) from HEK 293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 ug) from HEK 293T cells. A. Affinity purified rabbit anti-Sp1 antibody used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, using each antibody at 3.75 ug/mg lysate. Subsequently, was used at 0.04 ug/mL for WB. Detection by chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-232] - FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Affinity purified rabbit anti-Sp1 used at a dilution of 1:1,000 (1 ug/mL). Detection by DAB.
Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SP1 antibody (NB600-232) used for WB at 0.04 ug/mL. Detection by chemiluminescence with an exposure time of 3 minutes.
(1 Review)
(7 Publications)
Species: Hu, Mu
Applications: ChIP, IHC, IHC-P, IP, WB
Host cell factor
C2 Antibody (OTI5H9) ...
NBP2-71862
Western Blot: Host cell factor C2 Antibody (OTI5H9) - Azide and BSA Free [NBP2-71862] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Host cell factor C2.
Immunohistochemistry: Host cell factor C2 Antibody (OTI5H9) - Azide and BSA Free [NBP2-71862] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: Host cell factor C2 Antibody (OTI5H9) - Azide and BSA Free [NBP2-71862] - Analysis of Carcinoma of Human thyroid tissue.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
PSG1 [HRP]
DPSG10
(2 Publications)
Species: Hu
Applications: ELISA
COCO/DAND5
Antibody (OTI2H3)
NBP2-01223
Flow Cytometry: DAND5 Antibody (2H3) [NBP2-01223] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAND5 antibody, and then analyzed by flow cytometry.
Western Blot: DAND5 Antibody (2H3) [NBP2-01223] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAND5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAND5.
Immunohistochemistry-Paraffin: DAND5 Antibody (2H3) [NBP2-01223] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DAND5 mouse monoclonal antibody.
Species: Hu
Applications: Flow, IHC, IHC-P, WB
IL-2 [Unconjugated]
202-IL
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(26 Reviews)
(338 Publications)
Species: Hu
Applications: BA
AIBZIP Antibody
(3E3)
H00148327-M01
Proximity Ligation Assay: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of protein-protein interactions between CREB3 and CREB3L4. HeLa cells were stained with anti-CREB3 rabbit purified polyclonal 1:1200 and anti-CREB3L4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of monoclonal antibody to CREB3L4 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: AIBZIP Antibody (3E3) [H00148327-M01] - Analysis of CREB3L4 expression in transfected 293T cell line by CREB3L4 monoclonal antibody (M01), clone 3E3.Lane 1: CREB3L4 transfected lysate(43.4 KDa).Lane 2: Non-transfected lysate.
Species: Hu
Applications: ELISA, ICC/IF, KD, PLA, WB
Pit1 Antibody
NBP1-92273
Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273] - Staining of human pituitary gland shows moderate to strong nuclear positivity in neuroendocrine cells in the anterior lobe.
Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Pit1 Antibody [NBP1-92273] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
(15 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(36 Reviews)
(708 Publications)
Species: Hu
Applications: BA
hnRNP C1 + C2
Antibody (SN0652)
NBP2-67656
Flow Cytometry: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Calreticulin
Antibody - BSA Free
NB600-101
Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.
(26 Publications)
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB
Host Cell Factor
1/HCFC1 Antibody
NB100-68210
Host Cell Factor 1/HCFC1 Antibody
Species: Hu, Mu, SyHa
Applications: WB, Simple Western, IP

Publications for Host Cell Factor 1/HCFC1 Antibody (NB100-68210)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Review for Host Cell Factor 1/HCFC1 Antibody (NB100-68210) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-68210:
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Mouse 11/05/2009
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Summary

ApplicationWestern Blot
Sample TestedMouse Iiver tissue, Sample Amount: 50 ug
SpeciesMouse
LotA1
CommentsGood antibody for WB, IP and IF

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: 3% BSA in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP anti-rabbit IgG from Jaskson ImmunoResearch, Secondary Ab Dilution Ratio: 1/10000

Details

Detection NotesDetection Method: ECL, Exposure Time: 1 minute, Specific Bands: 100-210 kDa

Comments

CommentsGood antibody for WB, IP and IF

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FAQs for Host Cell Factor 1/HCFC1 Antibody (NB100-68210) (0)

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Secondary Antibodies

 

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Recent Reviews

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 Verified Customer
11/05/2009
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol HCFC1
Entrez
Uniprot