Host Cell Factor 1/HCFC1 Antibody

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.

Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY license.

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, SyHa
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Host Cell Factor 1/HCFC1 Antibody Summary

• Description: Novus Biologicals Rabbit Host Cell Factor 1/HCFC1 Antibody (NB100-68210) is a polyclonal antibody validated for use in WB, Simple Western and IP. Anti-Host Cell Factor 1/HCFC1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1985 and 2035 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).
• Predicted Species: Golden Syrian Hamster (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HCFC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Simple Western
  • Western Blot 1:2000-1:10000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Mouse ES cells and Human RPE cells, separated by Size

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Host Cell Factor 1/HCFC1 Antibody

• C1 Factor
• CFF
• CFFC1 factor
• HCF
• HCF1
• HCF1HFC1
• HCF-1VP16 accessory protein
• HCFC1
• HFC1
• host cell factor C1 (VP16-accessory protein)
• MGC70925
• VCAFhost cell factor 1

Background

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Host Cell Factor 1/HCFC1 Antibody (NB100-68210)(2)

Publications using NB100-68210

• Hsu J, Arand J, Chaikovsky A et al. E2F4 regulates transcriptional activation in mouse embryonic stem cells independently of the RB family Nat Commun 2019-07-03 [PMID: 31270324] (WB, Mouse)
• Yu H, Mashtalir N, Daou S et al. The ubiquitin carboxyl hydrolase BAP1 forms a ternary complex with YY1 and HCF-1 and is a critical regulator of gene expression. Mol Cell Biol 2010-11-01 [PMID: 20805357]

Review for Host Cell Factor 1/HCFC1 Antibody (NB100-68210) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 11/05/2009

Summary

• Application: Western Blot
• Sample Tested: Mouse Iiver tissue, Sample Amount: 50 ug
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: HCFC1
• Entrez:
  • 3054(Human)
• Uniprot:
  • NP_005325.2(Human)