Host Cell Factor 1/HCFC1 Antibody

Images

 
Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

Host Cell Factor 1/HCFC1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1985 and 2035 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HCFC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-68210 in the following applications:

Publications
Read Publication using NB100-68210.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Host Cell Factor 1/HCFC1 Antibody

  • C1 Factor
  • CFF
  • CFFC1 factor
  • HCF
  • HCF1
  • HCF1HFC1
  • HCF-1VP16 accessory protein
  • HCFC1
  • HFC1
  • host cell factor C1 (VP16-accessory protein)
  • MGC70925
  • VCAFhost cell factor 1

Background

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Host Cell Factor 1/HCFC1 Antibody (NB100-68210)(1)

Review for Host Cell Factor 1/HCFC1 Antibody (NB100-68210) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-68210:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 11/05/2009
View

Summary

ApplicationWestern Blot
Sample TestedMouse Iiver tissue, Sample Amount: 50 ug
SpeciesMouse
LotA1
CommentsGood antibody for WB, IP and IF

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: 3% BSA in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP anti-rabbit IgG from Jaskson ImmunoResearch, Secondary Ab Dilution Ratio: 1/10000

Details

Detection NotesDetection Method: ECL, Exposure Time: 1 minute, Specific Bands: 100-210 kDa

Comments

CommentsGood antibody for WB, IP and IF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Host Cell Factor 1/HCFC1 Antibody (NB100-68210) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Host Cell Factor 1/HCFC1 Antibody (NB100-68210)

Discover related pathways, diseases and genes to Host Cell Factor 1/HCFC1 Antibody (NB100-68210). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Host Cell Factor 1/HCFC1 Antibody (NB100-68210)

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PTMs for Host Cell Factor 1/HCFC1 Antibody (NB100-68210)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
11/05/2009
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HCFC1
Entrez
Uniprot