Hormone-sensitive Lipase/HSL Overexpression Lysate (Native)

Images

 
Western Blot: HSL Overexpression Lysate (Adult Normal) [NBL1-12548] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HSL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Hormone-sensitive Lipase/HSL Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209729 and based on accession number NM_005357. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens lipase, hormone-sensitive (LIPE), mRNA.
Gene
LIPE

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
116.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Hormone-sensitive Lipase/HSL

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Hormone-sensitive Lipase/HSL Overexpression Lysate (Native)

  • hormone-sensitive lipase testicular isoform
  • Hormonesensitive Lipase
  • Hormone-sensitive Lipase
  • HSL
  • HSLEC 3.1.1.79
  • LHS
  • lipase, hormone-sensitive
  • LIPE

Background

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Hormone-sensitive Lipase/HSL Lysate (NBL1-12548) (0)

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Bioinformatics

Gene Symbol LIPE
Entrez