Mouse Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL (example only; lot dependent)
• Sensitivity: 0.67 ng/mL (example only; lot dependent)

Mouse Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 1.56 - 100 ng/mL (example only; lot dependent)
• Sensitivity: 0.67 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LIPE

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

• hormone-sensitive lipase testicular isoform
• Hormonesensitive Lipase
• Hormone-sensitive Lipase
• HSL
• HSLEC 3.1.1.79
• LHS
• lipase, hormone-sensitive
• LIPE

Background

Hormone-sensitive lipase (HSL) is an intracellular neutral lipase found in a variety of tissues, but primarily in adipose and steroidogenic tissues. It hydrolyzes triglycerides into free fatty acids and cholesteryl esters into free cholesterol for steroid hormone synthesis.

HSL is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue, and HSL antibodies are useful tools for lipid and metabolism research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Hormone-sensitive Lipase/HSL ELISA Kit (NBP3-56067). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LIPE