Hormone-sensitive Lipase/HSL Antibody (729923) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 729923
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Hormone-sensitive Lipase/HSL Antibody (729923) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Hormone-sensitive Lipase/HSL
  Met302-Leu425
  Accession # Q05469
• Specificity: Detects human Hormone-sensitive Lipase/HSL in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hormone-sensitive Lipase/HSL Antibody (729923) [Alexa Fluor® 350]

• hormone-sensitive lipase testicular isoform
• Hormonesensitive Lipase
• Hormone-sensitive Lipase
• HSL
• HSLEC 3.1.1.79
• LHS
• lipase, hormone-sensitive
• LIPE

Background

HSL (hormone-sensitive lipase), also called LIPE, is an 84 kDa phosphoprotein that is the rate-limiting enzyme in the catalytic breakdown of triglycerides. The human 775 amino acid (aa) form is active in adipose tissue and skeletal muscle. Within the region used as an immunogen, human HSL shares 92% and 93% aa sequence identity with mouse and rat HSL, respectively. HSL translocates to triglyceride-metabolizing lipid droplets in response to epinephrine or contraction in skeletal muscle, but to the cytosol following insulin treatment of adipocytes. A 120 kDa isoform of human HSL with a 301 aa N-terminal extension is present in the testes, localized to elongating spermatids and spermatozoa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.