HNRNPA0 Antibody - BSA Free

Western Blot: HNRNPA0 Antibody [NBP2-22293] - HNRNPA0-knocked-down colorectal cancer cells showed inhibition of mitotic events and induction of apoptosis. Western blotting revealed that the expression of cleaved caspase-3 and PARP in HCT116 cells was increased by the downregulation of HNRNPA0 at 48 h. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2439-7), licensed under a CC-BY license.

Western Blot: HNRNPA0 Antibody [NBP2-22293] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. NBP2-22293 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation: HNRNPA0 Antibody [NBP2-22293] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22293 used for IP at 6 ug/mg lysate. hnRNP A0 was also immunoprecipitated by goat anti-hnRNP A0 antibody. For blotting immunoprecipitated hnRNP A0, NBP2-22293 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: HNRNPA0 Antibody [NBP2-22293] - hnRNP A0 maintained the alignment of chromosomes through the stabilization of RAB3GAP1 mRNA in cancer cells.Immunocytochemistry showed that abnormal spindle formation was detected in hnRNP A0 or RAB3GAP1-downregulated cells. Scale bar: 10 µm a. Western blotting revealed that Cyclin B1 & Securin were highly expressed in the cells treated with siRNA of HNRNP A0 or RAB3GAP1 (n = 3) b. RT-PCR confirmed the overexpression of ZWINT-1 in a colorectal cancer line (HCT116 cells c). A GEPIA analysis revealed the overexpression of mRNA of ZWINT-1 in colorectal cancer tissues d. Western blotting showed that the protein expression of RAB3GAP1 & ZWINT-1 was decreased in HNRNP A0-knockdown cells e (n = 3). RT-PCR showed that the mRNA expression of ZWINT-1 was not decreased in hnRNP A0 or RAB3GAP1-knockdown cells f. Western blotting revealed that the ZWINT-1 expression was decreased by the downregulation of hnRNP A0 & RAB3GAP1, & the degree of this decrease was reduced by treatment with MG132 g (n = 3). Immunocytochemistry showed that ZWINT-1 was co-localized with active rab3. Scale bar: 10 µm h. The number of tumors decreased by the downregulation of HNRNP A0 & RAB3GAP1 in AOM-DSS carcinogenesis model mice is shown i. The error bars & numbers show the S.D. *p < 0.05 by Student’s t-test & an ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32303675), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HNRNPA0 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HNRNPA0 Antibody - BSA Free (NBP2-22293) is a polyclonal antibody validated for use in WB and IP. Anti-HNRNPA0 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 150 to 200 of human Heterogeneous Nuclear Ribonucleoprotein A0 using the numbering given in entry NP_006796.1 (GeneID 10949).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPA0
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:1000 - 1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HNRNPA0 Antibody - BSA Free

• heterogeneous nuclear ribonucleoprotein A0
• hnRNA binding protein
• hnRNPA0
• HNRPA0hnRNP A0

Background

HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNRNPA0 Antibody (NBP2-22293)(2)

Publications using NBP2-22293

• Ting La, Song Chen, Xiao Hong Zhao, Shuai Zhou, Ran Xu, Liu Teng, Yuan Yuan Zhang, Kaihong Ye, Liang Xu, Tao Guo, Muhammad Fairuz Jamaluddin, Yu Chen Feng, Hai Jie Tang, Yanliang Wang, Qin Xu, Yue Gu, Huixia Cao, Tao Liu, Rick F. Thorne, Feng‐Min Shao, Xu Dong Zhang, Lei Jin LncRNA LIMp27 Regulates the DNA Damage Response through p27 in p53‐Defective Cancer Cells Advanced Science 2023-01-13 [PMID: 36638271]
• Konishi H, Fujiya M, Kashima S et al. A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells Cell Death Dis 2020-04-17 [PMID: 32303675] (WB, Human)

Bioinformatics

• Gene Symbol: HNRNPA0
• Uniprot:
  • NP_006796.1(Human)