HNRNPA0 Antibodies
We offer HNRNPA0 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each HNRNPA0 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HNRNPA0 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the HNRNPA0 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HNRNPA0 polyclonal antibodies and browse our HNRNPA0 monoclonal antibody catalog.
Alternate Names for HNRNPA0 Antibodies
anti-HNRNPA0 antibody, anti-heterogeneous nuclear ribonucleoprotein A0 antibody, anti-hnRNA binding protein antibody, anti-HNRPA0hnRNP A0 antibody
6 Results for "hnrnpa0" in Primary Antibodies
![Western Blot: HNRNPA0 Antibody [NBP1-57275] - Amount and Lane 1 : 5% Input Lane 2: 5% Sup Lane 3: Normal IgG Lane 4: hn-RNPA0 ppt. k562 Sample IP Antibody: HNRPA0 Amount of IP Antibody: Primary Antibody: HNRPA0 Primary Antibody Dilution: 1 : 4000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Western-Blot-NBP1-57275-img0008.jpg "Western Blot: HNRNPA0 Antibody [NBP1-57275] - Amount and Lane 1 : 5% Input Lane 2: 5% Sup Lane 3: Normal IgG Lane 4: hn-RNPA0 ppt. k562 Sample IP Antibody: HNRPA0 Amount of IP Antibody: Primary Antibody: HNRPA0 Primary Antibody Dilution: 1 : 4000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody")
![Immunocytochemistry/Immunofluorescence: HNRNPA0 Antibody [NBP1-57275] - MCF7 cells Primary Antibody Dilution : 1:200 Secondary Antibody: Anti-rabbit-FITC Secondary Antibody Dilution: 1:500 Color/Signal Descriptions: DAPI: Blue hn-RNPA0: Green Gene Name: HNRPA0](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-57275-img0006.jpg)
![Immunohistochemistry: HNRNPA0 Antibody [NBP1-57275] - Human cardiac cell Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunohistochemistry-NBP1-57275-img0007.jpg)
![Western Blot: HNRNPA0 Antibody [NBP1-57275] - Jurkat cell lysate, Antibody Titration: 0.625ug/ml](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Western-Blot-NBP1-57275-img0001.jpg)
![Western Blot: HNRNPA0 Antibody [NBP1-57275] - Lanes: Lane 1 : 20 ug HeLa S3 lysate Lane 2: 20 ug MCF7 lysate Lane 3: 20 ug K562 lysate Primary, Antibody Dilution: 1 : 4000 Secondary Antibody: Anti-rabbit-HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: HNRPA0.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Western-Blot-NBP1-57275-img0009.jpg)
![Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP1-57275] - Human Heart Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Myocardial cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunohistochemistry-Paraffin-NBP1-57275-img0002.jpg)
![Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP1-57275] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunohistochemistry-Paraffin-NBP1-57275-img0004.jpg)
![Western Blot: HNRNPA0 Antibody [NBP2-55847] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Western-Blot-NBP2-55847-img0003.jpg "Western Blot: HNRNPA0 Antibody [NBP2-55847] - Western blot analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: HNRNPA0 Antibody [NBP2-55847] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55847-img0007.jpg)
![Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunohistochemistry-Paraffin-NBP2-55847-img0012.jpg)
![Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunohistochemistry-Paraffin-NBP2-55847-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human cerebral cortex, endometrium, liver and lower gastrointestinal using Anti-HNRNPA0 antibody NBP2-55847 (A) shows similar protein distribution across tissues to independent antibody NBP1-83240 (B).](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunohistochemistry-Paraffin-NBP2-55847-img0009.jpg)
![Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human colon shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunohistochemistry-Paraffin-NBP2-55847-img0010.jpg)
![Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunohistochemistry-Paraffin-NBP2-55847-img0011.jpg)
![Immunohistochemistry: HNRNPA0 Antibody (PSH02-77) [NBP3-32439] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-HNRNPA0 antibody (NBP3-32439) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32439) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32439_rabbit-hnrnpa0-mab-psh02-77-2982024142093.jpg "Immunohistochemistry: HNRNPA0 Antibody (PSH02-77) [NBP3-32439] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-HNRNPA0 antibody (NBP3-32439) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32439) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: HNRNPA0 Antibody (PSH02-77) [NBP3-32439] - Immunocytochemistry analysis of 293T cells labeling HNRNPA0 with Rabbit anti-HNRNPA0 antibody (NBP3-32439) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HNRNPA0 antibody (NBP3-32439) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32439_rabbit-hnrnpa0-mab-psh02-77-29202410334097.jpg)
![Flow Cytometry: HNRNPA0 Antibody (PSH02-77) [NBP3-32439] - Flow cytometric analysis of 293T cells labeling HNRNPA0. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32439, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32439_rabbit-hnrnpa0-mab-psh02-77-392024884621.jpg)
![Western Blot: HNRNPA0 Antibody (PSH02-77) [NBP3-32439] - Western blot analysis of HNRNPA0 on different lysates with Rabbit anti-HNRNPA0 antibody (NBP3-32439) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A549 cell lysate Lane 4: Jurkat cell lysate Lane 5: A431 cell lysate Lane 6: COS-1 cell lysate Lane 7: PC-12 cell lysate Lane 8: Human brain tissue lysate Lane 9: Mouse brain tissue lysateLane 10: Mouse testis tissue lysateLane 11: Rat brain tissue lysate Lane 12: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 31 kDa Observed band size: 34/36 kDa Exposure time: Lane 1-12 (left): 10 seconds; Lane 1-12 (right): 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32439_rabbit-hnrnpa0-mab-psh02-77-3920248453548.jpg)
![Western Blot: HNRNPA0 Antibody [NBP2-22293] - HNRNPA0-knocked-down colorectal cancer cells showed inhibition of mitotic events and induction of apoptosis. Western blotting revealed that the expression of cleaved caspase-3 and PARP in HCT116 cells was increased by the downregulation of HNRNPA0 at 48 h. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2439-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Western-Blot-NBP2-22293-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HNRNPA0 Antibody [NBP2-22293] - HNRNPA0-knocked-down colorectal cancer cells showed inhibition of mitotic events and induction of apoptosis. Western blotting revealed that the expression of cleaved caspase-3 and PARP in HCT116 cells was increased by the downregulation of HNRNPA0 at 48 h. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2439-7), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: HNRNPA0 Antibody [NBP2-22293] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. NBP2-22293 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Western-Blot-NBP2-22293-img0002.jpg)
![Immunoprecipitation: HNRNPA0 Antibody [NBP2-22293] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22293 used for IP at 6 ug/mg lysate. hnRNP A0 was also immunoprecipitated by goat anti-hnRNP A0 antibody. For blotting immunoprecipitated hnRNP A0, NBP2-22293 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunoprecipitation-NBP2-22293-img0003.jpg)
![Western Blot: HNRNPA0 Antibody [NBP2-22293] - hnRNP A0 maintained the alignment of chromosomes through the stabilization of RAB3GAP1 mRNA in cancer cells.Immunocytochemistry showed that abnormal spindle formation was detected in hnRNP A0 or RAB3GAP1-downregulated cells. Scale bar: 10 µm a. Western blotting revealed that Cyclin B1 & Securin were highly expressed in the cells treated with siRNA of HNRNP A0 or RAB3GAP1 (n = 3) b. RT-PCR confirmed the overexpression of ZWINT-1 in a colorectal cancer line (HCT116 cells c). A GEPIA analysis revealed the overexpression of mRNA of ZWINT-1 in colorectal cancer tissues d. Western blotting showed that the protein expression of RAB3GAP1 & ZWINT-1 was decreased in HNRNP A0-knockdown cells e (n = 3). RT-PCR showed that the mRNA expression of ZWINT-1 was not decreased in hnRNP A0 or RAB3GAP1-knockdown cells f. Western blotting revealed that the ZWINT-1 expression was decreased by the downregulation of hnRNP A0 & RAB3GAP1, & the degree of this decrease was reduced by treatment with MG132 g (n = 3). Immunocytochemistry showed that ZWINT-1 was co-localized with active rab3. Scale bar: 10 µm h. The number of tumors decreased by the downregulation of HNRNP A0 & RAB3GAP1 in AOM-DSS carcinogenesis model mice is shown i. The error bars & numbers show the S.D. *p < 0.05 by Student’s t-test & an ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32303675), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22293_rabbit-polyclonal-hnrnpa0-antibody-3102024161223.jpg)
![Western Blot: HNRNPA0 Antibody [NBP3-13222] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HNRNPA0 antibody (NBP3-13222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Western-Blot-NBP3-13222-img0001.jpg "Western Blot: HNRNPA0 Antibody [NBP3-13222] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HNRNPA0 antibody (NBP3-13222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HNRNPA0 Antibody [NBP3-13222] - HNRNPA0 antibody detects HNRNPA0 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNRNPA0 stained by HNRNPA0 antibody (NBP3-13222) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/HNRNPA0-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13222-img0002.jpg)
![Immunoprecipitation: HNRNPA0 Antibody [NBP3-38221] - Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug HNRNPA0 antibody. Western blot was performed from the immunoprecipitate using HNRNPA0 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38221_rabbit-hnrnpa0-pab-101220241136050.jpg "Immunoprecipitation: HNRNPA0 Antibody [NBP3-38221] - Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug HNRNPA0 antibody. Western blot was performed from the immunoprecipitate using HNRNPA0 antibody at a dilution of 1:1000.")
![Western Blot: HNRNPA0 Antibody [NBP3-38221] - Western blot analysis of various lysates using HNRNPA0 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38221_rabbit-hnrnpa0-pab-1012202411404055.jpg)