hnRNP U Antibody

Western Blot: hnRNP U Antibody [NB100-2135] - Detection of Human and Mouse hnRNP-U on HeLa whole cell lysate using NB100-2135.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

hnRNP U Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 775 and the C-terminus (residue 824) of human Heterogeneous Nuclear Ribonucleoprotein U using the numbering given in entry NP_114032.1 (GeneID 3192).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPU
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Control:

  hnRNP U Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for hnRNP U Antibody

• heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• heterogeneous nuclear ribonucleoprotein U
• hnRNP U
• HNRPU
• p120 nuclear protein
• p120
• pp120
• SAFA
• SAF-AhnRNPU
• Scaffold attachment factor A
• U21.1

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hnRNP U Antibody (NB100-2135)(1)

Publication using NB100-2135

• Powell WT, Coulson RL, Crary FK et al. A Prader-Willi locus lncRNA cloud modulates diurnal genes and energy expenditure. Hum Mol Genet 2013-07-02 [PMID: 23771028] (WB, Mouse)

Bioinformatics

• Gene Symbol: HNRNPU
• Entrez:
  • 3192(Human)
  • 51810(Mouse)
• Uniprot:
  • Q00839(Human)