hnRNP U Antibody (3R3F1)

Western Blot: hnRNP U Antibody (3R3F1) [NBP3-16363] - Western blot analysis of extracts of various cell lines, using hnRNP U Rabbit mAb (NBP3-16363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug hnRNP U antibody (NBP3-16363). Western blot was performed from the immunoprecipitate using hnRNP U antibody (NBP3-16363) at a dilition of 1:1000.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Rat liver using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: hnRNP U Antibody (3R3F1) [NBP3-16363] - Confocal imaging of U-2 0S cells using hnRNP U Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Human colon using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Rat brain using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Human spleen using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: hnRNP U Antibody (3R3F1) [NBP3-16363] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using hnRNP U Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: 3R3F1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

hnRNP U Antibody (3R3F1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human hnRNP U (Q00839). MSSSPVNVKKLKVSELKEELKKRRLSDKGLKAELMERLQAALDDEEAGGRPAMEPGNGSLDLGGDSAGRSGAGLEQEAAAGGDEEEEEEEEEEEGISALD
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HNRNPU
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for hnRNP U Antibody (3R3F1)

• heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
• heterogeneous nuclear ribonucleoprotein U
• hnRNP U
• HNRPU
• p120 nuclear protein
• p120
• pp120
• SAFA
• SAF-AhnRNPU
• Scaffold attachment factor A
• U21.1

Background

hnRNP U belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPU