hnRNP A2B1 Antibody - BSA Free

Western Blot: hnRNP A2B1 Antibody [NBP2-16864] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: hnRNP A2B1 Antibody [NBP2-16864] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP A2B1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: hnRNP A2B1 Antibody [NBP2-16864] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Western Blot: hnRNP A2B1 Antibody [NBP2-16864] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:10000.

• Reactivity: Hu, Mu, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

hnRNP A2B1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.
• Localization: Nucleus; nucleoplasm; Cytoplasm (By similarity)
• Predicted Species: Mouse (90%), Rhesus Macaque (90%), Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPA2B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:20000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (89%), Xenopus laevis (83%), Chicken (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for hnRNP A2B1 Antibody - BSA Free

• DKFZp779B0244
• FLJ22720
• heterogeneous nuclear ribonucleoprotein A2/B1
• heterogeneous nuclear ribonucleoprotein B1
• heterogeneous nuclear ribonucleoproteins A2/B1
• hnRNP A2 / hnRNP B1
• HNRNPA2
• HNRNPB1
• HNRPA2
• HNRPA2B1hnRNP A2/B1
• HNRPB1
• nuclear ribonucleoprotein particle A2 protein
• RNPA2
• SNRPB1

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HNRNPA2B1