eIF3K Antibody

Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.

• Reactivity: Hu, ChHa, Mu, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

eIF3K Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 168 and 218 of human eukaryotic translation initiation factor 3, subunit K (eukaryotic translation initiation factor 3, subunit 12) using the numbering given in entry NP_037366.1 (
• Predicted Species: Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3K
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID:32839273).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF3K Antibody

• ARG134
• eIF3k
• EIF3-p28
• EIF3S12eIF-3 p28
• Eukaryotic translation initiation factor 3 subunit 12
• eukaryotic translation initiation factor 3 subunit K
• eukaryotic translation initiation factor 3, subunit 12
• eukaryotic translation initiation factor 3, subunit K
• HSPC029
• M9
• MSTP001
• muscle specific
• Muscle-specific gene M9 protein
• PLAC24
• PLAC-24
• PLAC-24eIF-3 p25
• PRO1474
• PTD001

Background

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF3K Antibody (NB100-93304)(6)

Publications using NB100-93304

• Duan H, Zhang S, Zarai Y et al. eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content The EMBO Journal 2023-06-15 [PMID: 37155573]
• Lee Y, Kim J, Kim MS et al. Coordinate regulation of the senescent state by selective autophagy Developmental cell 2021-04-27 [PMID: 33915088]
• Nakazawa K, Shichino Y, Iwasaki S, Shiina N Implications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation J. Biol. Chem. 2020-08-23 [PMID: 32839273] (WB, Chinese Hamster)
• Jeong SJ, Park S, Nguyen LT et al. A threonyl-tRNA synthetase-mediated translation initiation machinery Nat Commun 2019-03-22 [PMID: 30902983] (WB, Human)
• Lee AS, Kranzusch PJ, Cate JH. eIF3 targets cell-proliferation messenger RNAs for translational activation or repression Nature 2015-04-06 [PMID: 25849773] (WB, Human)
• Salsman J, Pinder J, Tse B et al. The translation initiation factor 3 subunit eIF3K interacts with PML and associates with PML nuclear bodies. Exp Cell Res 2013-10-15 [PMID: 24036361] (Human)

Bioinformatics

• Gene Symbol: EIF3K
• Entrez:
  • 27335(Human)
• Uniprot:
  • Q9UBQ5(Human)