HNF1 Antibody (4110) - Azide and BSA Free

Immunohistochemistry-Paraffin: HNF1 Antibody (4110) [NBP2-43652] - Sample: Paraffin-embedded human SW480. HNF1 alpha antibody (4110) diluted at 1:250.

Western Blot: HNF1 Antibody (4110) [NBP2-43652] - Analysis of 30 ug whole cell lysate. A: HepG2 7.5% SDS PAGE diluted at 1:1000.

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [GT4110] (NBP2-43652) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch
• Applications: WB, IHC
• Clone: 4110
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HNF1 Antibody (4110) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HNF1 Antibody (4110) - Azide and BSA Free (NBP2-43652) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HNF1. The exact sequence is proprietary.
• Predicted Species: Rat (99%), Porcine (99%), Bovine (98%), Chicken (95%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNF1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for HNF1 Antibody (4110) - Azide and BSA Free

• HNF1 homeobox A
• IDDM20
• LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor
• MODY3
• TCF1hepatic
• Transcription factor 1

Background

HNF1 is a transcription factor required for the expression of several liver-specific genes. The protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNF1A