HNF-6/ONECUT1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunochemical staining HNF-6/ONECUT1 in human hepatoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunocytochemistry/Immunofluorescence: HNF-6/ONECUT1 Antibody [NBP2-99314] - Immunofluorescence staining of HNF-6/ONECUT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99314 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus .

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HNF-6/ONECUT1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HNF-6/ONECUT1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ONECUT1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for HNF-6/ONECUT1 Antibody - Azide and BSA Free

• HNF6
• HNF-6
• HNF-6A
• HNF6Ahepatocyte nuclear factor 6, alpha
• HNF6hepatocyte nuclear factor 6
• HNF-6One cut domain family member 1
• OC-1
• one cut homeobox 1one cut domain, family member 1
• ONECUT1

Background

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ONECUT1