HNF-4 alpha/NR2A1 Antibody

Western Blot: HNF-4 alpha/NR2A1 Antibody [H00003172-D01P] - Analysis of HNF4A expression in Raw 264.7.

Western Blot: HNF-4 alpha/NR2A1 Antibody [H00003172-D01P] - Analysis of HNF4A expression in HeLa.

Western Blot: HNF-4 alpha/NR2A1 Antibody [H00003172-D01P] - Analysis of HNF4A expression in transfected 293T cell line by HNF4A polyclonal antibody.Lane 1: HNF4A transfected lysate(51.60 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: HNF-4 alpha/NR2A1 Antibody [H00003172-D01P] - Analysis of protein-protein interactions between HNF4A and CTNNB1. HeLa cells were stained with anti-HNF4A rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

HNF-4 alpha/NR2A1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: HNF4A (NP_849180.1, 1 a.a. - 464 a.a.) full-length human protein. MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMSTPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI
• Specificity: HNF4A - hepatocyte nuclear factor 4, alpha,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNF4A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HNF-4 alpha/NR2A1 Antibody

• FLJ39654
• hepatic nuclear factor 4 alpha
• hepatocyte nuclear factor 4, alpha
• hepatocyte nuclear factor 4-alpha
• HNF4 alpha
• HNF-4 alpha
• HNF4A
• HNF4a7
• HNF-4-alpha
• HNF4alpha10/11/12
• HNF4HNF4a8
• MODY
• MODY1
• NR2A1
• NR2A1HNF4a9
• NR2A21
• Nuclear receptor subfamily 2 group A member 1
• TCF
• TCF14
• TCF-14
• TCF14HNF4alpha
• Transcription factor 14
• Transcription factor HNF-4
• transcription factor-14

Background

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-4 alpha/NR2A1 Antibody (H00003172-D01P)(1)

Publication using H00003172-D01P

• Furumiya M, Inoue K, Ohta K et al. Transcriptional regulation of PCFT by KLF4, HNF4alpha, CDX2 and C/EBPalpha: implication in its site-specific expression in the small intestine. Biochem Biophys Res Commun. 2013-01-03 [PMID: 23313509]

Bioinformatics

• Gene Symbol: HNF4A
• Entrez:
  • 3172(Human)
• Uniprot:
  • NP_849180.1(Human)