HNF-4 alpha/NR2A1 Antibody (4E2)

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2. Lane 1: HNF4A transfected lysatE (51.6 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of monoclonal antibody to HNF4A on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi or non-transfected control. Blot probed with H00003172-M04. GAPDH (36.1 kDa) used as loading control.

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in HepG2 (Cat # L019V1).

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in Jurkat (Cat # L017V1).

ELISA: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Detection limit for recombinant GST tagged HNF4A is approximately 1ng/ml as a capture antibody.

Product Discontinued

HNF-4 alpha/NR2A1 Antibody (4E2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HNF4A (NP_000448, 324 a.a. ~ 423 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRP
• Specificity: HNF4A (4E2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNF4A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HNF-4 alpha/NR2A1 Antibody (4E2)

• FLJ39654
• hepatic nuclear factor 4 alpha
• hepatocyte nuclear factor 4, alpha
• hepatocyte nuclear factor 4-alpha
• HNF4 alpha
• HNF-4 alpha
• HNF4A
• HNF4a7
• HNF-4-alpha
• HNF4alpha10/11/12
• HNF4HNF4a8
• MODY
• MODY1
• NR2A1
• NR2A1HNF4a9
• NR2A21
• Nuclear receptor subfamily 2 group A member 1
• TCF
• TCF14
• TCF-14
• TCF14HNF4alpha
• Transcription factor 14
• Transcription factor HNF-4
• transcription factor-14

Background

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-4 alpha/NR2A1 Antibody (H00003172-M04)(1)

Publication using H00003172-M04

• Tsai WL, Yeh PH, Tsai CY et al. Efficient programming of human mesenchymal stem cell-derived hepatocytes by epigenetic regulations. J Gastroenterol Hepatol 2016-05-24 [PMID: 27218433]

Bioinformatics

• Gene Symbol: HNF4A
• Entrez:
  • 3172(Human)
• OMIM:
  • 125850(Human)
• Uniprot:
  • NP_000448(Human)