HNF-3 beta/FoxA2 Antibody (OTI3C10)

Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti HNF-3 beta/FoxA2 monoclonal antibody.(1:200).

Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Staining of HepG2 cells using anti HNF-3 beta/FoxA2 mouse monoclonal antibody at 1:50 dilution.

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Staining of paraffin-embedded lung using anti-FOXA2 mouse monoclonal antibody.

Flow Cytometry: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - HEK293T cells transfected with either pCMV6-ENTRY FOXA2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-FOXA2 mouse monoclonal, and then analyzed by flow cytometry.

Western Blot: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2.

Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2.

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (OTI3C10) [NBP2-02088] - Staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

HNF-3 beta/FoxA2 Antibody (OTI3C10) Summary

• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 48.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HNF-3 beta/FoxA2 Antibody (OTI3C10)

• forkhead box A2
• Forkhead box protein A2
• FoxA2
• hepatic nuclear factor-3-beta
• hepatocyte nuclear factor 3, beta
• hepatocyte nuclear factor 3-beta
• HNF3 beta
• HNF-3 beta
• HNF-3B
• HNF-3-beta
• HNF3BTCF-3B
• MGC19807
• TCF3B
• Transcription factor 3B

Background

FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-3 beta/FoxA2 Antibody (NBP2-02088)(3)

Publications using NBP2-02088

• Cao J, Yang Z. FOXA2 inhibits the TLR4/NF-kappa B signaling pathway and alleviates inflammatory activation of macrophages in rheumatoid arthritis by repressing LY96 transcription. Cytokine 2024-10-31 [PMID: 39486110]
• Liu X, Yan C, Chang C et al. FOXA2 Suppression by TRIM36 Exerts Anti-Tumor Role in Colorectal Cancer Via Inducing NRF2/GPX4-Regulated Ferroptosis Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-10-24 [PMID: 37875418] (ICC/IF, Human)
  
  Details:
  1:1000 ICC/IF dilution
• Rajderkar S, Mann JM, Panaretos C et al. Trim33 is required for appropriate development of pre-cardiogenic mesoderm Dev. Biol. 2019-03-30 [PMID: 30940539] (WB, Mouse)

Bioinformatics

• Gene Symbol: FOXA2
• Entrez:
  • 3170(Human)