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HAF017). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. TheE-RABPpromoter was detected by standard PCR." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue)." class="big_lightbox" target="_blank">
FOXA2 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.
![Western Blot HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-western-blot-2772023112644..jpg)
![Chromatin Immunoprecipitation (ChIP) HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/HNF-3_beta_AF2400_Chromatin_Immunoprecipitation_11227.jpg)
![Immunocytochemistry HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/HNF-3_beta_AF2400_Immunocytochemistry_6484.jpg)
![Immunohistochemistry HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/HNF3_beta_AF2400_Immunohistochemistry_23167.jpg)
![Simple Western HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-simple-western-639180079863195882.jpg)
![Immunocytochemistry HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-immunocytochemistry-2572023154638..jpg)
![Immunocytochemistry/ Immunofluorescence HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-41202410331941.jpg)
![Immunocytochemistry/ Immunofluorescence HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-236202594359..jpg)
![Flow Cytometry HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-flow-cytometry-12122025974218.jpg)
![Immunocytochemistry/ Immunofluorescence HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-12122025965717.jpg)
![Immunocytochemistry/ Immunofluorescence HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-121220259191715.jpg)
![Flow Cytometry HNF-3 beta/FoxA2 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2400_human-hnf-3-beta-foxa2-affinity-purified-polyclonal-ab-flow-cytometry-12122025961615.jpg)
