Human HMW Kininogen ELISA Kit (Colorimetric)

ELISA: Human HMW Kininogen ELISA Kit (Colorimetric) [NBP2-60479] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell Culture, Urine, Saliva, Milk, CSF
• Standard Curve Range: 0.391 - 25 ng/mL (example only; lot dependent)
• Sensitivity: 1.5 ng/ml (example only; lot dependent)

Human HMW Kininogen ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.391 - 25 ng/mL (example only; lot dependent)
• Sensitivity: 1.5 ng/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.20% (example only; lot dependent)
• Intra-Assay: CV% < 4.90% (example only; lot dependent)
• Spike Recovery: 96.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: Kng1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human HMW Kininogen ELISA Kit (Colorimetric)

• Alpha-2-thiol proteinase inhibitor
• BDK
• bradykinin
• Fitzgerald factor
• High molecular weight kininogen
• kininogen 1
• kininogen
• kininogen-1
• KNGHMWK
• Williams-Fitzgerald-Flaujeac factor

Background

High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein (see MIM 147910)-kinin system. The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing. Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3. However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L. In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide. BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin (see MIM 601699) from endothelial cells. During vascular damage, BK stimulates smooth muscle proliferation and intimal hypertrophy. Release of BK from HMWK generates a 2-chain HMWK, termed HMWKa, containing the heavy and light chains joined by a disulfide bond (Merkulov et al., 2008 [PubMed 18000168]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: Kng1