HMGB1/HMG-1 Antibody (19N12A1) - BSA Free

Western Blot: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Analysis using HMGB1 antibody. A) Full-length human HMGB1 protein, B) human HeLa cell lysate and C) mouse NIH 3T3 cell lysate were probed with HMGB1 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Image shows a specific band for HMGB1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow
• Clone: 19N12A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HMGB1/HMG-1 Antibody (19N12A1) - BSA Free Summary

• Immunogen: Full-length recombinant human HMGB1 was used as the immunogen.
• Specificity: This antibody recognizes both B box domain (amino acids 88-162) and full length HMGB1 protein.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 29601597)
  • Simple Western 1:100
  • Western Blot 1 - 3 ug/mL
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 35 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HMGB1/HMG-1 Antibody (19N12A1) - BSA Free

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMGB1/HMG-1 Antibody (NBP2-27400)(1)

Publication using NBP2-27400

• Coleman LG Jr, Maile R, Jones SW et al. HMGB1/IL-1beta complexes in plasma microvesicles modulate immune responses to burn injury. PLoS One 2018-03-30 [PMID: 29601597] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: HMGB1
• Uniprot:
  • P09429()