HMGB1/HMG-1 Antibody (19N10B7) [Biotin]

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, ChHa, Eq, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 19N10B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

HMGB1/HMG-1 Antibody (19N10B7) [Biotin] Summary

• Immunogen: Full-length recombinant human HMGB1 protein
• Localization: Nucleus (very high), Cytoplasm (very low)
• Specificity: This antibody recognizes both A box domain (amino acids 9-79) and full length HMGB1 protein.
• Predicted Species: Rat (99%), Porcine (99%), Bovine (99%), Chicken (91%), Canine (100%), Equine (100%), Primate (100%), Chinese Hamster (99%). Backed by our 100% Guarantee.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Based upon immunogen sequence similarity, this antibody is predicted to react with Rat (99%), Bovine (99%), Porcine/Pig (99%), Chinese Hamster (99%), Chicken (91%), Canine/Dog (100%), Primate/Monkey (100%) and Equine/Horse (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HMGB1/HMG-1 Antibody (19N10B7) [Biotin]

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGB1