HMGA2 Overexpression Lysate (Native)

Images

 
Western Blot: HMGA2 Overexpression Lysate (Adult Normal) [NBL1-11613] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HMGA2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HMGA2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC214629 and based on accession number NM_003483. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens high mobility group AT-hook 2 (HMGA2), transcript variant 1, mRNA.
Gene
HMGA2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HMGA2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HMGA2 Overexpression Lysate (Native)

  • BABL
  • BABLHMGI-C
  • high mobility group AT-hook 2
  • High mobility group AT-hook protein 2
  • high mobility group protein HMGI-C
  • high-mobility group (nonhistone chromosomal) protein isoform I-C
  • High-mobility group protein HMGI-C
  • HMGA2
  • HMGIC
  • HMGICSTQTL9
  • LIPO

Background

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HMGA2 Lysate (NBL1-11613) (0)

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Video Protocols

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Bioinformatics

Gene Symbol HMGA2
Entrez