HMGA2 Antibody

Western Blot: HMGA2 Antibody [25810002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil Target weight [kDa]: 16, 12, 11 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Western Blot: HMGA2 Antibody [25810002] - Analysis of HMGA2 in HeLa whole cell lysate. Image courtesy of anonymous customer product review.

ELISA: HMGA2 Antibody [25810002]

Product Discontinued

HMGA2 Antibody Summary

• Immunogen: This antibody is specific for the entire range of the target protein.
• Specificity: This product is specific for Human HMGA2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMGA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.
This product may not be purchased or sold within the European Union. Please contact customer service with any questions.

Alternate Names for HMGA2 Antibody

• BABL
• BABLHMGI-C
• high mobility group AT-hook 2
• High mobility group AT-hook protein 2
• high mobility group protein HMGI-C
• high-mobility group (nonhistone chromosomal) protein isoform I-C
• High-mobility group protein HMGI-C
• HMGA2
• HMGIC
• HMGICSTQTL9
• LIPO

Background

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMGA2 Antibody (25810002)(3)

Publications using 25810002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Kim Th, Song Jy, Park H et al. miR-145, targeting high-mobility group A2, is a powerful predictor of patient outcome in ovarian carcinoma. Cancer Lett. 2014-11-10 [PMID: 25444913] (WB, Human)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Review for HMGA2 Antibody (25810002) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Wei-Ting Lu WB Human 06/22/2012

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: t00447-a1

Bioinformatics

• Gene Symbol: HMGA2
• Entrez:
  • 8091(Human)
• Uniprot:
  • P52926()