HLA G Antibody

Images

 
Western Blot: HLA G Antibody [NBP2-16855] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-G antibody diluted at 1:500.
Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Paraffin-embedded human lung cancer. HLA-G antibody diluted at 1:500.
Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Paraffin-embedded human endometrial cancer. HLA-G antibody diluted at 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.63 mg/ml

Order Details

HLA G Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HLA-G. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HLA-G
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.63 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HLA G Antibody

  • b2 microglobulin
  • HLA class I histocompatibility antigen, alpha chain G
  • HLA class I molecule
  • HLA G antigen
  • HLA G
  • HLA-6.0
  • HLA-G histocompatibility antigen, class I, G
  • HLAG
  • HLA-G
  • major histocompatibility complex, class I, G
  • MHC Class I Antigen G
  • MHC-G

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Blogs on HLA G.

HLA G - mediating immune tolerance during pregnancy
Human leukocyte antigen G (HLA G) is a major histocompatibility complex (MHC) class I molecule that is primarily expressed in the placenta and is essential for the immune tolerance of the fetus during pregnancy. Unlike many HLA genes, HLA G has re...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-G
Entrez
Uniprot