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![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. HLA-G immunostaining (brown color) indicates the location of extravillous trophoblasts. In control explants (left panel) almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA (0.25nM, right panel). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-55650-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0005.jpg "Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. HLA-G immunostaining (brown color) indicates the location of extravillous trophoblasts. In control explants (left panel) almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA (0.25nM, right panel). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-55650-5) licensed under a CC-BY license.")
![Western Blot: HLA G Antibody (MEM-G/1) [NB500-302] - Using the Biotin direct conjugate analysis (reducing conditions) of HLA-G1 in HLA-G1 transfectants using the antibody MEM-G/1 biotin.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Western-Blot-NB500-302-img0003.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1B - first-trimester placenta (paraffin-embedded sections)](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0001.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1. Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary disseases (paraffin-embedded sections) The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0002.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) - BSA Free [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. (a) HLA-G immunostaining (brown color top panel) indicates the location of extravillous trophoblasts. PhosphoH3(Ser10) immunostaining (brown color lower 3 panels) identifies proliferating cells undergoing mitosis. In extravillous trophoblasts of explants treated with ELA proliferation appears to decrease. In control explants almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA. Treatment with ELA in combination with ML221 does not return villous cytotrophoblast proliferation to the level as seen in control explants. (b) Quantifying the percentage of proliferating extravillous trophoblasts observed by immunohistochemistry shows a significant decrease upon treatment with ELA which cannot be rescued by the addition of ML221. P = 0.08 between controls & explants treated with ELA & ML221. n = 12 placentas, 5 explants per treatment. Bars represent mean ± SEM & data was tested with one-way ANOVA followed by Bonferroni multiple comparisons test. *Indicates p < 0.05. (c) Treatment of explants with an ELA neutralizing antibody shows a significant increase of extravillous trophoblast proliferation upon quantifying immunohistochemistry stainings. n = 4 placentas, 5 explants per treatment. Bars represent mean ± SEM & data was tested with a Student’s t-test. *Indicates p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31836787), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-302_mouse-monoclonal-hla-g-antibody-mem-g-1-31020241617149.jpg)
![Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunocytochemistry-Immunofluorescence-NB500-314-img0006.jpg "Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/9) [NB500-314] - Staining of HLA-G1 transfectants (LCL-HLA-G1) using Alexa Fluor 488 conjugated anti-human HLA-G (MEM-G/9).")
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0012.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of APC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0010.jpg)
![Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Flow-Cytometry-NB500-314-img0011.jpg)
![Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9, NB500-314) and protein G. HLA-G was detected by anti-human HLA-G (4H84) and goat anti-mouse HRP in cell lysate (Lanes 1) and in the immunoprecipitate (Lanes 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-9-Immunoprecipitation-NB500-314-img0009.jpg)
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0004.jpg "Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.")
![Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) purified, GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0001.jpg)
![Flow Cytometry: HLA G Antibody (01G) [NBP1-44926] - Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) FITC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-Flow-Cytometry-NBP1-44926-img0002.jpg)
![Flow Cytometry: HLA G Antibody (01G) - BSA Free [NBP1-44926] - Surface staining pattern of HLA-G transfected LCL cells using anti-human HLA-G (01G) purified antibody (concentration in sample 16 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-01G-BSA-Free-Flow-Cytometry-NBP1-44926-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).](http://images.novusbio.com/fullsize/HLA-G-Antibody-4H84-Immunohistochemistry-Paraffin-NB110-55297-img0003.jpg "Immunohistochemistry-Paraffin: HLA G Antibody (4H84) [NB110-55297] - Staining of human spleen (paraffin sections) using anti-HLA-G (4H84).")
![Western Blot: HLA G Antibody (4H84) [NB110-55297] - Analysis of HLA-G in HLA-G1 transfectants (LCL-HLA-G1) by 4H84 antibody-biotin.](http://images.novusbio.com/fullsize/HLA-G-Antibody-4H84-Western-Blot-NB110-55297-img0002.jpg)
![Western Blot: HLA G Antibody (4H84) [NB110-55297] - HLA G Antibody (4h84) [NB110-55297] - Immunoprecipitation of HLA-G from HLA-G1 transfectanted lysates (LCL-HLA-G1) by MEM-G/9 antibody and protein G. HLA-G was detected by 4H84 antibody (NB110-55297) and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).](http://images.novusbio.com/fullsize/HLA-G-Antibody-4h84-Immunoprecipitation-NB110-55297-img0001.jpg)
![Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Western-Blot-NBP1-44924-img0001.jpg "Western Blot: HLA G Antibody (5A6G7) [NBP1-44924] - Analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector Lane 2: M8 cell line transfected with HLA-G1 Lane 3: M8 cell line transfected with HLA-G5 Lane 4: M8 cell line transfected with HLA-G6")
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0003.jpg)
![Flow Cytometry: HLA G Antibody (5A6G7) [NBP1-44924] - Staining of HLA-G in HLA-G6 transfectants with anti-HLA-G (5A6G7) PE.](http://images.novusbio.com/fullsize/HLA-G-Antibody-5A6G7-Flow-Cytometry-NBP1-44924-img0002.jpg)
![Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).](http://images.novusbio.com/fullsize/HLA-G-Antibody-2A12-Flow-Cytometry-NBP1-44925-img0002.jpg "Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells, red and orange) with anti-HLA-G (2A12) FITC, compared with nontransfected cells (blue, green).")
![Flow Cytometry: HLA G Antibody (2A12) [NBP1-44925] - Surface staining of HLA-G (on transfected cells) with anti-HLA-G (2A12) purified, GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-2A12-Flow-Cytometry-NBP1-44925-img0001.jpg)
![Western Blot: HLA G Antibody [NBP2-16855] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-G antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HLA-G-Antibody-Western-Blot-NBP2-16855-img0005.jpg "Western Blot: HLA G Antibody [NBP2-16855] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-G antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Human lung cancer. HLA-G antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Mahlarvu xenograft, using HLA-G antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0001.jpg)
![Immunohistochemistry-Paraffin: HLA G Antibody [NBP2-16855] - Human endometrial cancer. HLA-G antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HLA-G-Antibody-Immunohistochemistry-Paraffin-NBP2-16855-img0002.jpg)
![Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-4-Western-Blot-NB500-533-img0001.jpg "Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).")
![Flow Cytometry: HLA G Antibody (G233) [NB110-55298] - Surface staining of HLA-G in HLA-G transfectants using anti-HLA-G (G233) purified / GAM-APC.](http://images.novusbio.com/fullsize/HLA-G-Antibody-G233-Flow-Cytometry-NB110-55298-img0001.jpg "Flow Cytometry: HLA G Antibody (G233) [NB110-55298] - Surface staining of HLA-G in HLA-G transfectants using anti-HLA-G (G233) purified / GAM-APC.")
![Immunocytochemistry /Immunofluorescence: HLA G Antibody (mem-G/9) - BSA Free [NBP3-43587] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with NBP3-43587 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43587, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43587_rabbit-hla-g-mab-mem-g-9-azide-and-bsa-free-74202522113046.png "Immunocytochemistry /Immunofluorescence: HLA G Antibody (mem-G/9) - BSA Free [NBP3-43587] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with NBP3-43587 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43587, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.")