HLA DQ/DR/DP Antibody (WR18)

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Flow Cytometry: HLA DQ/DR/DP Antibody (WR18) [NB100-64358] - Staining of human peripheral blood lymphocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clone
WR18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HLA DQ/DR/DP Antibody (WR18) Summary

Immunogen
Human HLA Class II (DP, DQ, DR)
Specificity
Recognizes HLA Class II beta chains of human major histocompatability antigen, Mw 28 kD. It reacts with a monomorphic determinant common to each beta chain type. The antigen is expressed by human B lymphocytes, monocytes and activated T lymphocytes.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DQA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Use in ICC/IF reported in secitific publication PMID: 32451495
Publications
Read Publication using
NB100-64358 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HLA DQ/DR/DP Antibody (WR18)

  • CD
  • CELIAC1DQ alpha 1 chain
  • DC-1 alpha chain
  • DQ-A1
  • FLJ27088
  • FLJ27328
  • GSE
  • HLA class II histocompatibility antigen, DQ(W3) alpha chain
  • HLA-DCA
  • HLA-DQA
  • leucocyte antigen DQA1
  • leukocyte antigen alpha chain
  • major histocompatibility complex, class II, DQ alpha 1
  • MGC149527
  • MHC class II antigen
  • MHC class II DQA1
  • MHC class II HLA-D alpha glycoprotein
  • MHC class II HLA-DQ-alpha-1
  • MHC class II surface glycoprotein
  • MHC HLA-DQ alpha

Background

The major histocompatibility complex (MHC) is a large genomic region or gene family found in most vertebrates containing many genes with important immune system roles. In humans, the MHC spans almost 4 megabases of chromosome 6 and includes more than 200 known genes, of which about half have known immmunological functions. The best known genes in the MHC region are the subset that encodes cell-surface antigen-presenting proteins. In humans, these genes are referred to as human leukocyte antigen (HLA) genes. The most intensely studied HLA genes are: HLA A, HLA B, HLA C, HLA DPA, HLA DPB1, HLA DQA1, HLA DQB1, HLA DRA, and HLA DRB1. In humans, the HLA is divided into three regions: Class I, II, and III. The A, B, and C genes belong to HLA class I while the six D genes belong to class II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA DQ/DR/DP Antibody (NB100-64358)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Filter By Species
Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for HLA DQ/DR/DP Antibody (NB100-64358) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-64358AF350
Alexa Fluor 405 NB100-64358AF405
Alexa Fluor 488 NB100-64358AF488
Alexa Fluor 532 NB100-64358AF532
Alexa Fluor 594 NB100-64358AF594
Alexa Fluor 647 NB100-64358AF647
Alexa Fluor 700 NB100-64358AF700
Alexa Fluor 750 NB100-64358AF750
Allophycocyanin NB100-64358APC
Allophycocyanin/Cy7 NB100-64358APCCY7
Biotin NB100-64358B
DyLight 350 NB100-64358UV
DyLight 405 NB100-64358V
DyLight 488 NB100-64358G
DyLight 550 NB100-64358R
DyLight 594 NB100-64358DL594
DyLight 650 NB100-64358C
DyLight 680 NB100-64358FR
DyLight 755 NB100-64358IR
FITC NB100-64358F
HRP NB100-64358H
Janelia Fluor 549 NB100-64358JF549
Janelia Fluor 646 NB100-64358JF646
PE NB100-64358PE
PE/Atto594 NB100-64358PEATT594
PE/Cy5.5 NB100-64358PECY55
PE/Cy7 NB100-64358PECY7
PerCP NB100-64358PCP

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Bioinformatics

Gene Symbol HLA-DQA1