HLA DQ/DR/DP Antibody (WR18) - BSA Free

Flow Cytometry: HLA DQ/DR/DP Antibody (WR18) [NB100-64358] - Staining of human peripheral blood lymphocytes.

• Reactivity: Hu
• Applications: Flow, Func, IHC
• Clone: WR18
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HLA DQ/DR/DP Antibody (WR18) - BSA Free Summary

• Description: Novus Biologicals Mouse HLA DQ/DR/DP Antibody (WR18) - BSA Free (NB100-64358) is a monoclonal antibody validated for use in IHC, Flow, ICC/IF and IP. Anti-HLA DQ/DR/DP Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human HLA Class II (DP, DQ, DR)
• Specificity: Recognizes HLA Class II beta chains of human major histocompatability antigen, Mw 28 kD. It reacts with a monomorphic determinant common to each beta chain type. The antigen is expressed by human B lymphocytes, monocytes and activated T lymphocytes.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DQA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Use in ICC/IF reported in scientific publication PMID: 32451495.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HLA DQ/DR/DP Antibody (WR18) - BSA Free

• CD
• CELIAC1DQ alpha 1 chain
• DC-1 alpha chain
• DQ-A1
• FLJ27088
• FLJ27328
• GSE
• HLA class II histocompatibility antigen, DQ(W3) alpha chain
• HLA-DCA
• HLA-DQA
• leucocyte antigen DQA1
• leukocyte antigen alpha chain
• major histocompatibility complex, class II, DQ alpha 1
• MGC149527
• MHC class II antigen
• MHC class II DQA1
• MHC class II HLA-D alpha glycoprotein
• MHC class II HLA-DQ-alpha-1
• MHC class II surface glycoprotein
• MHC HLA-DQ alpha

Background

The major histocompatibility complex (MHC) is a large genomic region or gene family found in most vertebrates containing many genes with important immune system roles. In humans, the MHC spans almost 4 megabases of chromosome 6 and includes more than 200 known genes, of which about half have known immmunological functions. The best known genes in the MHC region are the subset that encodes cell-surface antigen-presenting proteins. In humans, these genes are referred to as human leukocyte antigen (HLA) genes. The most intensely studied HLA genes are: HLA A, HLA B, HLA C, HLA DPA, HLA DPB1, HLA DQA1, HLA DQB1, HLA DRA, and HLA DRB1. In humans, the HLA is divided into three regions: Class I, II, and III. The A, B, and C genes belong to HLA class I while the six D genes belong to class II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA DQ/DR/DP Antibody (NB100-64358)(5)

Publications using NB100-64358

• O'Connell BC, Hubbard C, Zizlsperger N et Al. Eganelisib combined with immune checkpoint inhibitor therapy and chemotherapy in frontline metastatic triple-negative breast cancer triggers macrophage reprogramming, immune activation and extracellular matrix reorganization in the tumor microenvironment J Immunother Cancer 2024-01-01 [PMID: 39214650]
• Kendall, TJ;Jimenez-Ramos, M;Turner, F;Ramachandran, P;Minnier, J;McColgan, MD;Alam, M;Ellis, H;Dunbar, DR;Kohnen, G;Konanahalli, P;Oien, KA;Bandiera, L;Menolascina, F;Juncker-Jensen, A;Alexander, D;Mayor, C;Guha, IN;Fallowfield, JA; An integrated gene-to-outcome multimodal database for metabolic dysfunction-associated steatotic liver disease Nature medicine 2023-10-30 [PMID: 37903863]
• Hartman K, Steiner G, Siegel M et al. Expanding the MAPPs Assay to Accommodate MHC-II Pan Receptors for Improved Predictability of Potential T Cell Epitopes Biology (Basel) 2023-09-21 [PMID: 37759665] (Immunoprecipitation)
• Liu Y, Yang J, Hilliard TS et al. Host obesity alters the ovarian tumor immune microenvironment and impacts response to standard of care chemotherapy Journal of experimental & clinical cancer research : CR 2023-07-12 [PMID: 37438818] (Immunohistochemistry-Paraffin, Human)
• Bockorny B, Semenisty V, Macarulla T et al. BL-8040, a CXCR4 antagonist, in combination with pembrolizumab and chemotherapy for pancreatic cancer: the COMBAT trial Nat. Med. 2020-05-25 [PMID: 32451495] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: HLA-DQA1