HLA DQ/DR/DP Antibody (CR3/43)

Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (CR3/43) [NB120-17101] - Formalin fixed paraffin embedded human tonsil stained with HLA-DR antibody (NB120-17101).

• Reactivity: Hu
• Applications: IHC
• Clone: CR3/43
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HLA DQ/DR/DP Antibody (CR3/43) Summary

• Immunogen: Tonsil cells
• Localization: Cell Membrane
• Specificity: This antibody reacts with the beta chain of all products of the gene sub regions DP, DQ, and DR. It stains B cells, interdigitating reticulum cells, Langerhans cells and many macrophages. This antibody does not react with normal T cell and polymorphs but stains activated T cells.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DQA1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Paraffin 1:25-1:50
• Application Notes: IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 30 min at RT.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for HLA DQ/DR/DP Antibody (CR3/43)

• CD
• CELIAC1DQ alpha 1 chain
• DC-1 alpha chain
• DQ-A1
• FLJ27088
• FLJ27328
• GSE
• HLA class II histocompatibility antigen, DQ(W3) alpha chain
• HLA-DCA
• HLA-DQA
• leucocyte antigen DQA1
• leukocyte antigen alpha chain
• major histocompatibility complex, class II, DQ alpha 1
• MGC149527
• MHC class II antigen
• MHC class II DQA1
• MHC class II HLA-D alpha glycoprotein
• MHC class II HLA-DQ-alpha-1
• MHC class II surface glycoprotein
• MHC HLA-DQ alpha

Background

The major histocompatibility complex (MHC) is a large genomic region or gene family found in most vertebrates containing many genes with important immune system roles. In humans, the MHC spans almost 4 megabases of chromosome 6 and includes more than 200 known genes, of which about half have known immmunological functions. The best known genes in the MHC region are the subset that encodes cell-surface antigen-presenting proteins. In humans, these genes are referred to as human leukocyte antigen (HLA) genes. The most intensely studied HLA genes are: HLA A, HLA B, HLA C, HLA DPA, HLA DPB1, HLA DQA1, HLA DQB1, HLA DRA, and HLA DRB1. In humans, the HLA is divided into three regions: Class I, II, and III. The A, B, and C genes belong to HLA class I while the six D genes belong to class II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA DQ/DR/DP Antibody (NB120-17101)(1)

Publication using NB120-17101

• Hosoya M, Kitama T, Shimanuki MN et al. Distribution of macrophages in the developing cochlea of the common marmoset, a primate model animal Frontiers in Immunology 2023-08-22 [PMID: 37675123] (Immunohistochemistry)

Bioinformatics

• Gene Symbol: HLA-DQA1
• Entrez:
  • 3119(Human)
• Uniprot:
  • P01920(Human)