Home » HLA DQ » HLA DQ Antibodies » HLA DQ Antibody (TAL 4.1)

HLA DQ Antibody (TAL 4.1)

 Submit a Review

Images

 
There are currently no images for HLA DQ Antibody (NB100-2700).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
TAL 4.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-2700AF405
Alexa Fluor 488
NB100-2700AF488
Alexa Fluor 647
NB100-2700AF647
Alexa Fluor 700
NB100-2700AF700
Allophycocyanin
NB100-2700APC
Biotin
NB100-2700B
DyLight 350
NB100-2700UV
DyLight 405
NB100-2700V
DyLight 488
NB100-2700G
DyLight 550
NB100-2700R
DyLight 650
NB100-2700C
DyLight 680
NB100-2700FR
DyLight 755
NB100-2700IR
FITC
NB100-2700F
HRP
NB100-2700H
Janelia Fluor 549
NB100-2700JF549
Janelia Fluor 646
NB100-2700JF646
PE
NB100-2700PE
PerCP
NB100-2700PCP
100% Guarantee on every product

HLA DQ Antibody (TAL 4.1) Summary

Immunogen
Whole cells from the B line WT46
Localization
Cell Membrane
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DQA1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Adrenal Lysate (NB820-60567)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for HLA DQ Antibody (TAL 4.1)

  • CD
  • CELIAC1DQ alpha 1 chain
  • DC-1 alpha chain
  • DQ-A1
  • FLJ27088
  • FLJ27328
  • GSE
  • HLA class II histocompatibility antigen, DQ(W3) alpha chain
  • HLA-DCA
  • HLA-DQA
  • leucocyte antigen DQA1
  • leukocyte antigen alpha chain
  • major histocompatibility complex, class II, DQ alpha 1
  • MGC149527
  • MHC class II antigen
  • MHC class II DQA1
  • MHC class II HLA-D alpha glycoprotein
  • MHC class II HLA-DQ-alpha-1
  • MHC class II surface glycoprotein
  • MHC HLA-DQ alpha

Background

Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens: class I (HLA A, HLA B and HLA C) and class II (HLA D).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(21 Reviews)
(527 Publications)
Species: Hu
HAI-2/SPINT2
Antibody [Unconjugate
AF1107
HAI-2/SPINT2 Antibody [Unconjugated]
(2 Publications)
Species: Mu
Applications: WB, IHC
Histatin 3
Antibody (4G9)
H00003347-M01
Immunocytochemistry/Immunofluorescence: Histatin 3 Antibody (4G9) [H00003347-M01] - Analysis of monoclonal antibody to HTN3 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: Histatin 3 Antibody (4G9) [H00003347-M01] - Detection limit for recombinant GST tagged HTN3 is approximately 3ng/ml as a capture antibody.
(1 Publication)
Species: Hu
Applications: ELISA, ICC/IF
B7-1/CD80 Antibody
(37711) [Unconjugated ...
MAB140
Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).
B7‑1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Human B7‑1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND50 is typically 0.2-1 µg/mL.
(6 Publications)
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(2 Reviews)
(1 Publication)
Species: Hu
IL-2 [Unconjugated]
202-IL
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
(13 Reviews)
(244 Publications)
Species: Hu
B7-2/CD86
[Unconjugated]
9090-B2
When Recombinant Human B7-2/CD86 (9090-B2) is coated at 0.5 µg/mL, Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) binds with a typical ED50 of7-42 ng/mL.
Species: Hu
CD4 Antibody
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
(7 Reviews)
(14 Publications)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
CD34 Antibody (MEC
14.7)
NB600-1071
Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
(18 Publications)
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Mouse CD3+ T Cell,
Small Column Enrichme ...
MTCC-10-NOV
Mouse CD3+ T Cell, Small Column Enrichment Kit
CD8 alpha Antibody
(53-6.7)
NBP1-49045
Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.
Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
(2 Reviews)
(11 Publications)
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
IL-6 [Unconjugated]
7270-IL/CF
IL-6 [Unconjugated]
Species: Hu
CD55/DAF Antibody
[Unconjugated]
AF2009
CD55/DAF was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 15 µg/mL Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CD55/DAF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (left panel, positive staining) and MOLT-4 human acute lymphoblastic leukemia cell line (right panel, negative staining) using Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(3 Publications)
Species: Hu
Applications: IHC, ICC
IL-4
6507-IL/CF
IL-4
(2 Publications)
Species: Hu
CD21 Antibody
(SC0681)
NBP2-67605
Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on different lysates using anti-CD21 antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: Raji
Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
CD28 Antibody
(37407) [Unconjugated
MAB342
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 0.2-0.6 μg/mL.
(25 Publications)
Species: Hu
Applications: WB, AgAct
CD45 Antibody
(30-F11)
NB100-77417
Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of FFPE section of mouse spleen using rat monoclonal CD45 antibody (clone 30-F11) at 1:50 dilution. The antibody generated an expected membrane staining in the red and white pulp spleenocytes of the tested spleen section.
Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody clone 30-F11 at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. Image submitted via a verified customer review.
Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with CD45 antibody (NB100-77417) at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).
(4 Reviews)
(19 Publications)
Species: Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
Calretinin Antibody
NBP1-32244
Western Blot: Calretinin Antibody [NBP1-32244] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE Calretinin antibody, antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-32244] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calretinin protein stained by Calretinin antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen Section adult mouse hippocampus. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
CRIPTO Antibody
NB100-1598
Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with CRIPTO Antibody NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
(2 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF
DHRS4 Antibody
NBP1-83790
Western Blot: DHRS4 Antibody [NBP1-83790] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: DHRS4 Antibody [NBP1-83790] - Staining of human cell line A-431 shows positivity in nuclear membrane and vesicles.
Immunohistochemistry-Paraffin: DHRS4 Antibody [NBP1-83790] - Staining of human kidney shows strong cytoplasmic positivity in tubule cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for HLA DQ Antibody (NB100-2700) (0)

There are no publications for HLA DQ Antibody (NB100-2700).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HLA DQ Antibody (NB100-2700) (0)

There are no reviews for HLA DQ Antibody (NB100-2700). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HLA DQ Antibody (NB100-2700) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Human Adrenal Membrane Tissue Lysate (Adult Membrane Normal)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NB100-2700

Bioinformatics Tool for HLA DQ Antibody (NB100-2700)

Discover related pathways, diseases and genes to HLA DQ Antibody (NB100-2700). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HLA DQ Antibody (NB100-2700)

Discover more about diseases related to HLA DQ Antibody (NB100-2700).
 

Pathways for HLA DQ Antibody (NB100-2700)

View related products by pathway.

PTMs for HLA DQ Antibody (NB100-2700)

Learn more about PTMs related to HLA DQ Antibody (NB100-2700).
 

Research Areas for HLA DQ Antibody (NB100-2700)

Find related products by research area.

Blogs on HLA DQ

There are no specific blogs for HLA DQ, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HLA DQ Antibody (TAL 4.1) and receive a gift card or discount.


Bioinformatics

Gene Symbol HLA-DQA1
Entrez