HLA Class I Antibody (W6/32) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
W6/32
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

HLA Class I Antibody (W6/32) [Alexa Fluor® 750] Summary

Immunogen
Membranes from human tonsillar lymphocytes
Specificity
Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HLA-A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA Class I Antibody (W6/32) [Alexa Fluor® 750]

  • A-10 alpha chain
  • FLJ26655
  • HLA class I histocompatibility antigen, A-1 alpha chain
  • HLA class I histocompatibility antigen, A-28 alpha chain
  • HLA class I histocompatibility antigen, A-9 alpha chain
  • HLA Class I
  • HLAA
  • HLA-A
  • HLA-ABC
  • major histocompatibility complex, class I, A
  • MHC class I antigen A*1
  • MHC class I antigen A*11
  • MHC class I antigen A*80

Background

HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.
  1. Barnstable, C.J. et al. (1978) Cell 14:9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HLA-A