HLA Class I Antibody (W6/32) [PerCP (Peridinin-chlorophyll Protein Complex)]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I PerCP-conjugated Monoclonal Antibody (Catalog # FAB7098C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: W6/32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP (Peridinin-chlorophyll Protein Complex)

HLA Class I Antibody (W6/32) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: Membranes from human tonsillar lymphocytes
• Specificity: Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA Class I Antibody (W6/32) [PerCP (Peridinin-chlorophyll Protein Complex)]

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLA Class I
• HLAA
• HLA-A
• HLA-ABC
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

The MHC (Major Histocompatibility Complex) is a group of at least 200 genes located on chromosome 6 in human. It contains multiple groupings, one of which is called Class I that contains three distinct, but closely related molecules. These three molecules are known as MHC Class I-A, -B, and -C which, in the human, have been renamed HLA (Human Leukocyte Antigen)-A, -B, and -C. All are 44-46 kDa type I transmembrane glycoproteins that share approximately 85% amino acid sequence identity in their extracellular domains. And all represent the alpha - component of a alpha - beta heterodimer that utilizes the 11-12 kDa transmembrane beta 2-microglobulin protein as a beta -component. These HLA heterodimers appear on all nucleated cells, and serve as a platform for the presentation of cytoplasmic components (both self and foreign) to the alpha beta -TCR of cytotoxic CD8+ T cells. Unedited or mutated "self" components should be ignored, while tumor or viral components should elicit a cytotoxic immune response. This requires the continuous internal "processing" or degradation of large proteins into 8-10 amino acid peptides that are subsequently bound to a type A, B or C heterodimer and cycled to the plasma membrane. On the cell surface, the B chain is most common while the C chain is least common. And with advancing age, both the A and B chains decline in number. Not all A, B and C chains are "engaged"; while 90% of the HLA B chains are associated with antigen, only 30-70% of A and C chains are associated with processed antigens. And identical peptides can be perceived differently. For instance, a nine amino acid peptide with O-linked (but not N-linked) glycosylation will not be recognized by a CD8+ T cell that is specific for the naked nine amino acid peptide. Finally, the A, B and C chains, if not the entire heterodimeric complex, are now known to act in-cis with LILRB2, generating an activating complex on select cell types. The mouse MHC counterpart to the human HLA system is called H-2, and the two mouse genes that correspond to human HLA-A and -B show 68% amino acid sequence identity over their entire lengths.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-A