HLA Class I Antibody (W6/32) [Allophycocyanin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I APC-conjugated Monoclonal Antibody (Catalog # FAB7098A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: W6/32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

HLA Class I Antibody (W6/32) [Allophycocyanin] Summary

• Immunogen: Membranes from human tonsillar lymphocytes
• Specificity: Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA Class I Antibody (W6/32) [Allophycocyanin]

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLA Class I
• HLAA
• HLA-A
• HLA-ABC
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.

1. Barnstable, C.J. et al. (1978) Cell 14:9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA Class I Antibody (FAB7098A)(1)

Publication using FAB7098A

• MA Lawson, FH Ebetino, A Mazur, AD Chantry, J Paton-Houg, HR Evans, D Lath, MK Tsoumpra, MW Lundy, RL Dobson, M Quijano, AA Kwaasi, JE Dunford, X Duan, JT Triffitt, G Jeans, RG Russell The Pharmacological Profile of a Novel Highly Potent Bisphosphonate, OX14 (1-fluoro-2-(imidazo-[1,2-?]pyridin-3-yl)ethyl-bisphosphonate J. Bone Miner. Res, 2017-04-21;0(0):. 2017-04-21 [PMID: 28337806] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: HLA-A