HLA Class I Antibody (W6/32) [Alexa Fluor® 700]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7098N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: W6/32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

HLA Class I Antibody (W6/32) [Alexa Fluor® 700] Summary

• Immunogen: Membranes from human tonsillar lymphocytes
• Specificity: Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA Class I Antibody (W6/32) [Alexa Fluor® 700]

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLA Class I
• HLAA
• HLA-A
• HLA-ABC
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

The MHC (Major Histocompatibility Complex) is a group of at least 200 genes located on chromosome 6 in human. It contains multiple groupings, one of which is called Class I that contains three distinct, but closely related molecules. These three molecules are known as MHC Class I-A, -B, and -C which, in the human, have been renamed HLA (Human Leukocyte Antigen)-A, -B, and -C. All are 44-46 kDa type I transmembrane glycoproteins that share approximately 85% amino acid sequence identity in their extracellular domains. And all represent the alpha - component of a alpha - beta heterodimer that utilizes the 11-12 kDa transmembrane beta 2-microglobulin protein as a beta -component. These HLA heterodimers appear on all nucleated cells, and serve as a platform for the presentation of cytoplasmic components (both self and foreign) to the alpha beta -TCR of cytotoxic CD8+ T cells. Unedited or mutated "self" components should be ignored, while tumor or viral components should elicit a cytotoxic immune response. This requires the continuous internal "processing" or degradation of large proteins into 8-10 amino acid peptides that are subsequently bound to a type A, B or C heterodimer and cycled to the plasma membrane. On the cell surface, the B chain is most common while the C chain is least common. And with advancing age, both the A and B chains decline in number. Not all A, B and C chains are "engaged"; while 90% of the HLA B chains are associated with antigen, only 30-70% of A and C chains are associated with processed antigens. And identical peptides can be perceived differently. For instance, a nine amino acid peptide with O-linked (but not N-linked) glycosylation will not be recognized by a CD8+ T cell that is specific for the naked nine amino acid peptide. Finally, the A, B and C chains, if not the entire heterodimeric complex, are now known to act in-cis with LILRB2, generating an activating complex on select cell types. The mouse MHC counterpart to the human HLA system is called H-2, and the two mouse genes that correspond to human HLA-A and -B show 68% amino acid sequence identity over their entire lengths.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-A