HLA A Antibody (22.63.4)

Product Discontinued

HLA A Antibody (22.63.4) Summary

• Immunogen: Nalm-6 cell line.
• Specificity: This antibody reacts with a common framework determinant of the 40-44kD protein of HLA-ABC Class I antigen. The antibody precipitates antigens of 40 - 44kD and 12kD (b2-microglobulin). Cell reactivity: The antibody reacts with all cells having HLA-ABC surface antigen. Erythrocytes and K562 and DAUDI are negative.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:15 - 1:60
  • Immunocytochemistry/ Immunofluorescence 1:15 - 1:60
  • Immunohistochemistry 1:10-1:500
• Application Notes: This antibody is suitable for indirect immunofluorescence analysis by flow cytometry and is an excellent positive control. It is suitable for cryostat sections but not for routine formalin fixed paraffin sections. If the tissue is prepared at 4C and fixed in PLP (Periodate-Lysine-Paraformaldehyde) before paraffin embedding, the antigen can be conserved (Hancock, 1982). SUGGESTED USAGE :DILUTION 1. Flow cytometry and indirect immunofluorescence: Dilute with isotonic buffer. Use 50 ul of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 ul buffer. 2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:15 will be applicable to most commonly used systems.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 50% (v/v) Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_002116.5

Alternate Names for HLA A Antibody (22.63.4)

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLAA
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-A
• Uniprot:
  • P16189(Human)