Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti-Histone H3 Antibody (NB500-171) used at 1:20,000 and Anti-Histone H4 used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Histone H4 Antibody [NBP2-16848] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-16848] - Human breast cancer, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Western Blot: Histone H4 Antibody [NBP2-16848] - WB for total histone in MCF-7 and HCT116 cells. Image from verified customer review.

Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 15% SDS PAGE diluted at 1:10000

Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:10000

Dot Blot: Histone H4 Antibody [NBP2-16848] - Peptide samples (0.1 ug) were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17) antibody Histone H4 at different dilution as indicated. A: Peptide samples of Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17). B: Peptide samples of Histone H4

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, DB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16). The exact sequence is proprietary.
• Modification: ac Lys5, ac Lys8, ac Lys12, ac Lys16
• Localization: Nucleus
• Predicted Species: Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIST4H4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Application Notes: Dot blot Assay-dependent dilution. Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] antibody validated for WB from a verified customer review
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody

• H4
• H4/A
• H4/B
• H4/C
• H4/D
• H4/E
• H4/G
• H4/H
• H4/I
• H4/J
• H4/K
• H4/M
• H4/N
• H4/O
• H4/p
• H4F2
• H4FA
• H4FB
• H4FC
• H4FD
• H4FE
• H4FG
• H4FH
• H4FI
• H4FJ
• H4FK
• H4FM
• H4FN
• H4FO
• H4M
• HIST1H4A
• HIST1H4B
• HIST1H4C
• HIST1H4D
• HIST1H4E
• HIST1H4F
• HIST1H4H
• HIST1H4I
• HIST1H4J
• HIST1H4K
• HIST1H4L
• HIST2H4
• HIST2H4A
• HIST2H4B
• HIST4H4
• histone 4, H4
• Histone Cluster 1 H4
• Histone Cluster 1 H4i
• Histone Cluster 4 H4
• histone cluster 4, H4
• Histone H4
• MGC24116

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Histone H4 Antibody (NBP2-16848)(2)

Publications using NBP2-16848

• Anderzhanova, E, Hafner, K Et al. The stress susceptibility factor FKBP51 controls S-ketamine-evoked release of mBDNF in the prefrontal cortex of mice. Neurobiol Stress 2020-11-01 [PMID: 33344695] (WB, Mouse)
• Lowe DJ, Herzog M, Mosler T et al. Chronic irradiation of human cells reduces histone levels and deregulates gene expression Sci Rep 2020-02-10 [PMID: 32042076] (WB, Human)

Review for Histone H4 Antibody (NBP2-16848) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Smita Singh WB Human 07/25/2014

Summary

• Application: Western Blot
• Sample Tested: MCF-7 and HCT116 cells
• Species: Human

Bioinformatics

• Gene Symbol: HIST4H4