Histone H4 Antibody

Images

 
Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:10000
Immunocytochemistry/ Immunofluorescence: Histone H4 Antibody [NBP2-16848] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by ...read more
Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-16848] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.
Western Blot: Histone H4 Antibody [NBP2-16848] - WB for total histone in MCF-7 and HCT116 cells. Image from verified customer review.
Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 15% SDS PAGE diluted at 1:10000
Dot Blot: Histone H4 Antibody [NBP2-16848] - Peptide samples (0.1 ug) were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17) antibody Histone H4 at different ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, DB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Histone H4 Antibody Summary

Immunogen
The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Xenopus (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Dot Blot
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Dot blot Assay-dependent dilution
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-16848 in the following applications:

Reactivity Notes

Xenopus laevis (100% homology).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H4 Antibody

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H4 Antibody (NBP2-16848) (0)

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Review for Histone H4 Antibody (NBP2-16848) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-16848:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Histone H4 NBP2-16848
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reviewed by:
Anonymous
WB Human 07/25/2014
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Summary

ApplicationWestern Blot
Sample TestedMCF-7 and HCT116 cells
SpeciesHuman
CommentsThis antibody gives a faint band above the desired band when probed from whole cell lyaste and a single band when a acid extracted histone prep is used.

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:2000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 20 sec

Comments

CommentsThis antibody gives a faint band above the desired band when probed from whole cell lyaste and a single band when a acid extracted histone prep is used.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NBP2-16848) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Histone H4 Products

Bioinformatics Tool for Histone H4 Antibody (NBP2-16848)

Discover related pathways, diseases and genes to Histone H4 Antibody (NBP2-16848). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Histone H4 Antibody (NBP2-16848)

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Pathways for Histone H4 Antibody (NBP2-16848)

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PTMs for Histone H4 Antibody (NBP2-16848)

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Research Areas for Histone H4 Antibody (NBP2-16848)

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIST4H4
Entrez