Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - WB analysis of H4K16ac in 1. HeLa histone prep lysate and 2. NIH 3T3 histone prep lysate.
Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys16] Antibody [NB21-2077] - Histone H4 K16ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Dot Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Dot blot analysis of H4K16ac in picomoles of protein.
This H4K16ac antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. In Western Blot, a band is seen ~13kDa in HeLa histone prep and NIH 3T3 histone prep. In ICC/IF, nuclear staining was observed in HeLa cells. This antibody is not applicable for Chromatin Immunoprecipitation.
Human, mouse and rat. Predicted to react with most mammal species.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
EpiPlus antibodies are produced in collaboration with Rockland Immunochemicals Inc.
Alternate Names for Histone H4 [ac Lys16] Antibody
histone 4, H4
Histone Cluster 1 H4
Histone Cluster 1 H4i
Histone Cluster 4 H4
histone cluster 4, H4
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys16 (H4 K16Ac) plays a role in transcriptional activation and DNA repair. The protein MOF (MYST1) is the primary enzyme to acetylate K16, along with Gcn5, Esal, ATF2, and Sas2. Depletion of MOF reduces K16 acetylation, causing cell cycle arrests, chromosomal aberrations, and a poor DNA damage response.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Sample Information: Cell Line or Tissue: Ovary Species: Pig Treatment: None. Run on control tissue
Controls: Positive Control: None Negative Control: Primary only, secondary only, and secondary with IgG all negative Loading Control (please attach additional images if applicable): Ponceau S stain
Total Protein Loaded: 40 ug/well
Electrophoresis: Gel Percentage: Gradient, 4-20% Voltage: 50V then 90V Time: 5 min then 1 hr
Membrane Transfer: Method (Submersion/Semi-dry): Semi-dry using iBlot2 system Membrane Type (PVDF/Nitrocellulose): Nitrocellulose Time: 7 min (iBlot2 Protocol 0) Voltage: Click here to enter text.
Histone H4: Implications in Liver Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post.
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