Histone H4 [ac Lys16] Antibody

Images

 
Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - WB analysis of H4K16ac in 1. HeLa histone prep lysate and 2. NIH 3T3 histone prep lysate.
Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys16] Antibody [NB21-2077] - Histone H4 K16ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Dot Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Dot blot analysis of H4K16ac in picomoles of protein.
Histone H4 [ac Lys16] Antibody [NB21-2077]

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, DB, ICC/IF, ChIP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.62 mg/ml

Histone H4 [ac Lys16] Antibody Summary

Immunogen
Synthetic acetylated peptide surrounding Lysine 16 of human Histone H4 [Swiss Prot P62805].
Modification
ac Lys16
Localization
Nucleus. Chromosome.
Marker
H4k16ac
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This H4K16ac antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. In Western Blot, a band is seen ~13kDa in HeLa histone prep and NIH 3T3 histone prep. In ICC/IF, nuclear staining was observed in HeLa cells. This antibody is not applicable for Chromatin Immunoprecipitation.
Positive Control
HeLa Lysate (NBP1-78155)
NIH 3T3 Lysate (NBP1-78157)
Reviewed Applications
Read 1 Review rated 5
using
NB21-2077 in the following applications:

Reactivity Notes

Human, mouse and rat. Predicted to react with most mammal species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.62 mg/ml
Purity
Immunogen affinity purified

Notes

EpiPlus antibodies are produced in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H4 [ac Lys16] Antibody

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4K16ac
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys16 (H4 K16Ac) plays a role in transcriptional activation and DNA repair. The protein MOF (MYST1) is the primary enzyme to acetylate K16, along with Gcn5, Esal, ATF2, and Sas2. Depletion of MOF reduces K16 acetylation, causing cell cycle arrests, chromosomal aberrations, and a poor DNA damage response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H4 Antibody (NB21-2077) (0)

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Review for Histone H4 Antibody (NB21-2077) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Pig.

Reviews using NB21-2077:
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Images Ratings Applications Species Date Details
Western Blot Histone H4 NB21-2077
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reviewed by:
Anonymous
WB Pig 11/17/2016
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Summary

ApplicationWestern Blot
Sample TestedOvary tissue
SpeciesPig

Comments

CommentsSample Information:
     Cell Line or Tissue: Ovary
     Species: Pig
     Treatment: None. Run on control tissue

Controls:
     Positive Control: None
     Negative Control: Primary only, secondary only, and secondary with IgG all negative
     Loading Control (please attach additional images if applicable): Ponceau S stain

Total Protein Loaded: 40 ug/well

Electrophoresis:
Gel Percentage: Gradient, 4-20%
     Voltage: 50V then 90V
     Time: 5 min then 1 hr

Membrane Transfer:
     Method (Submersion/Semi-dry): Semi-dry using iBlot2 system
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 7 min (iBlot2 Protocol 0)
     Voltage: Click here to enter text.

Blocking:
     Blocking Solution: 5% BSA in.2% PBST
     Time: 1.5 hours

Primary Antibody:
     Dilution: 1:1000
     Diluent Buffer: 5% BSA in.2% PBST     
     Incubation Time: overnight     
     Incubation Temperature: 4°C

Washing Conditions:
     Wash Solution:.2% PBST
     Time and Repetitions: 3X, 10 minutes each

Secondary Antibody
Manufacturer and Catalog #: Cell Signaling, #7074
Secondary description: goat anti-rabbit
     Dilution: 1:1000
     Diluent Buffer: 5% BSA in.2% PBST
     Incubation Time: 1 hour
     Incubation Temperature: room temperature

Detection Method:
Detection: SignalFire ECL reagent (Cell Signaling)
Procedure: ECL on blot 3 min
Development Time: 5 min

Molecular weight of band(s): 13 kDa

Product General Protocols

View specific protocols for Histone H4 Antibody (NB21-2077): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NB21-2077) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-2077AF405
Alexa Fluor 488 Labeled NB21-2077AF488
Alexa Fluor 647 Labeled NB21-2077AF647
Alexa Fluor 700 Labeled NB21-2077AF700
Biotin Labeled NB21-2077B
DyLight 405 Labeled NB21-2077V
DyLight 488 Labeled NB21-2077G
DyLight 550 Labeled NB21-2077R
DyLight 650 Labeled NB21-2077C
FITC Labeled NB21-2077F
HRP Labeled NB21-2077H

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Recent Reviews

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1
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Anonymous
11/17/2016
Application: WB
Species: Pig

Bioinformatics

Gene Symbol HIST4H4
Entrez
Uniprot