Histone H4 [ac Lys5] Antibody

Images

 
Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - WB analysis of H4K5ac in NIH 3t3 histone prep.
Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized ...read more
Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - WB analysis of H4K5ac in HeLa histone prep.
Histone H4 [ac Lys5] Antibody [NB21-2024]

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.78 mg/ml

Histone H4 [ac Lys5] Antibody Summary

Immunogen
Synthetic aceylated peptide surrounding Lysine 5 of human Histone H4 [Swiss Prot P62805].
Modification
ac Lys5
Localization
Nucleus. Chromosome
Marker
H4K5ac
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This Histone H4 K5ac antibody is useful for ChIP, Dot blot, ICC/IF and Western blot where a band is seen ~13kDa.
Positive Control
HeLa Lysate (NBP1-78155)
NIH 3T3 Lysate (NBP1-78157)
Reviewed Applications
Read 2 Reviews rated 5
using
NB21-2024 in the following applications:

Publications
Read Publication using NB21-2024.

Reactivity Notes

Human, mouse and rat. Predicted to react with most mammal species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.78 mg/ml
Purity
Immunogen affinity purified

Notes

EpiPlus antibodies are produced in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H4 [ac Lys5] Antibody

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4K5ac
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H4 Antibody (NB21-2024)(1)

We have publications tested in 1 application: WB.


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WB
(1)
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Reviews for Histone H4 Antibody (NB21-2024) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Pig.

Reviews using NB21-2024:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Pig
(1)
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Images Ratings Applications Species Date Details
Western Blot Histone H4 NB21-2024
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reviewed by:
Anonymous
WB Pig 11/17/2016
View

Summary

ApplicationWestern Blot
Sample TestedOvary tissue
SpeciesPig

Comments

CommentsTreatment: None. Run on control tissue

Controls
Positive Control: None
Negative Control: Primary only, secondary only, and secondary with IgG all negative
Loading Control: Ponceau S stain

Total Protein Loaded: 40 ug/well

Electrophoresis:
Gel Percentage: Gradient, 4-20%
Electrophoresis Conditions:
     Voltage: 50V then 90V
     Time: 5 min then 1 hr

Membrane Transfer:
     Method (Submersion/Semi-dry): Semi-dry using iBlot2 system
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 7 min (iBlot2 Protocol 0)
     Voltage: Click here to enter text.

Blocking:
     Blocking Solution: 5% BSA in.2% PBST
     Time: 2.5 hours

Primary Antibody:
     Dilution: 1:1000
     Diluent Buffer: 5% BSA in.2% PBST     
     Incubation Time: overnight     
     Incubation Temperature: 4°C

Washing Conditions:
     Wash Solution:.2% PBST
     Time and Repetitions: 3X, 10 minutes each

Secondary Antibody
Manufacturer and Catalog #: Cell Signaling, #7074
Secondary description: goat anti-rabbit
     Dilution: 1:1000
     Diluent Buffer: 5% BSA in.2% PBST
     Incubation Time: 1 hour
     Incubation Temperature: room temperature

Detection Method:
Detection: SignalFire ECL reagent (Cell Signaling)
Procedure: ECL on blot 3 min
Development Time: 5 min

Molecular weight of band(s): 13 kDa
 
reviewed by:
Anonymous
WB Human 10/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:2000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol

Product General Protocols

View specific protocols for Histone H4 Antibody (NB21-2024): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NB21-2024) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-2024AF405
Alexa Fluor 488 Labeled NB21-2024AF488
Alexa Fluor 647 Labeled NB21-2024AF647
Alexa Fluor 700 Labeled NB21-2024AF700
Biotin Labeled NB21-2024B
DyLight 405 Labeled NB21-2024V
DyLight 488 Labeled NB21-2024G
DyLight 550 Labeled NB21-2024R
DyLight 650 Labeled NB21-2024C
FITC Labeled NB21-2024F
HRP Labeled NB21-2024H

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Bioinformatics Tool for Histone H4 Antibody (NB21-2024)

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
11/17/2016
Application: WB
Species: Pig

 
Anonymous
10/04/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIST4H4
Entrez
Uniprot