Histone H4 [Monomethyl Arg3] Antibody

Images

 
Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - WB analysis of Histone H4 R3me1 in NIH/3T3 histone preps.
Immunocytochemistry/ Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Histone H4 R3me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.43 mg/ml

Histone H4 [Monomethyl Arg3] Antibody Summary

Immunogen
Synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4 [Swiss Prot P62805].
Modification
Monomethyl Arg3
Localization
Nucleus. Chromosome
Marker
H4R3me1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This H4R3me1 antibody is useful for ChIP*, Immunocytochemistry/Immunofluorescence, Dot Blot, and Western Blot where a band is seen ~13kDa.
Control Peptide
Histone H4 Peptide (NB21-2011PEP)
Publications
Read Publication using
NB21-2011 in the following applications:

Reactivity Notes

Human, mouse and rat. Predicted to react with most mammal species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.43 mg/ml
Purity
Immunogen affinity purified

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc. *Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.

Alternate Names for Histone H4 [Monomethyl Arg3] Antibody

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4M
  • H4R3Me1
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

Histone H4 is an 11.4 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. Histone H4 shows transcriptional regulation that is both cell cycle-dependent and -independent. Histone H4 is modified by phosphorylation, acetylation, ribosylation, and methylation. Histone H4 has been shown to interact with other histone acetytransferases, histone deacetylases, and PARP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H4 Antibody (NB21-2011)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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ChIP
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NB21-2011) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-2011AF405
Alexa Fluor 488 Labeled NB21-2011AF488
Alexa Fluor 647 Labeled NB21-2011AF647
Alexa Fluor 700 Labeled NB21-2011AF700
Biotin Labeled NB21-2011B
DyLight 405 Labeled NB21-2011V
DyLight 488 Labeled NB21-2011G
DyLight 550 Labeled NB21-2011R
DyLight 650 Labeled NB21-2011C
FITC Labeled NB21-2011F
HRP Labeled NB21-2011H

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST4H4
Entrez
Uniprot