Western Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in HeLa histone prep.
Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in nonmitotic and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin were ...read more
Chromatin Immunoprecipitation: Histone H4 [p Ser1] Antibody [NB21-2000] - 2 ug of NB21-2000 was used to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to ...read more
Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in in nonmitotic, prophase, and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin ...read more
Dot Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in picomoles of peptide.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Alternate Names for Histone H4 [p Ser1] Antibody
histone 4, H4
Histone Cluster 1 H4
Histone Cluster 1 H4i
Histone Cluster 4 H4
histone cluster 4, H4
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of H4 Ser1 (H4 pS1) has been linked to mitosis and DNA repair. This modification is enriched in sites proximal to double stranded brakes, but not those associated with UV damage. Casein kinase II (CK2) phosphorylates H4 Ser1, and it also implicated in regulating the DNA damage response. Furthermore, recruitment of CK2 requires the SIN3/RPD3 histone deacetylase complex.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Histone H4: Implications in Liver Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post.
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