Histone H4 [p Ser1] Antibody

Images

 
Western Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - WB analysis of Histone H4 pS1 in HeLa histone prep.
Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in nonmitotic and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin were ...read more
Chromatin Immunoprecipitation: Histone H4 [p Ser1] Antibody [NB21-2000] - 2 ug of NB21-2000 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to ...read more
Immunocytochemistry/ Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in in nonmitotic, prophase, and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin ...read more
Dot Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Dot blot analysis of Histone H4 pS1 in picomoles of peptide.
Histone H4 [p Ser1] Antibody [NB21-2000]

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.89 mg/ml

Histone H4 [p Ser1] Antibody Summary

Immunogen
Synthetic phosphorylated peptide surrounding Serine 1 of human Histone H4 [Swiss Prot P62805].
Modification
p Ser1
Localization
Nucleus.
Marker
H4pS1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 0.1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This H4pS1 antibody is useful for ICC/IF, ChIP, Dot blot, and Western blot where a band is detected ~13 kDa.
Control
HeLa Lysate (NB820-59462)
Control Peptide
Histone H4 Peptide (NB21-2000PEP)
Publications
Read Publications using NB21-2000.

Reactivity Notes

Human, mouse and rat. Predicted to react with most mammal species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.89 mg/ml
Purity
Immunogen affinity purified

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H4 [p Ser1] Antibody

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4M
  • H4S1p
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of H4 Ser1 (H4 pS1) has been linked to mitosis and DNA repair. This modification is enriched in sites proximal to double stranded brakes, but not those associated with UV damage. Casein kinase II (CK2) phosphorylates H4 Ser1, and it also implicated in regulating the DNA damage response. Furthermore, recruitment of CK2 requires the SIN3/RPD3 histone deacetylase complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H4 Antibody (NB21-2000)(2)

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Product General Protocols

View specific protocols for Histone H4 Antibody (NB21-2000): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NB21-2000) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-2000AF405
Alexa Fluor 488 Labeled NB21-2000AF488
Alexa Fluor 647 Labeled NB21-2000AF647
Alexa Fluor 700 Labeled NB21-2000AF700
Biotin Labeled NB21-2000B
DyLight 405 Labeled NB21-2000V
DyLight 488 Labeled NB21-2000G
DyLight 550 Labeled NB21-2000R
DyLight 650 Labeled NB21-2000C
FITC Labeled NB21-2000F
HRP Labeled NB21-2000H

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST4H4
Entrez
Uniprot