Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, Dr, Ma, YeSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
5E10-D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [Biotin] Summary

Immunogen
Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805]
Modification
Monomethyl Lys20
Localization
Nucleus
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HIST4H4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Histone H4 [Monomethyl Lys20] (5E10-D8) antibody is useful in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence and Western blot. In WB a band is seen at ~11 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

All mammals including human, mouse and rat, Drosophila, C. elegans, yeast.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [Biotin]

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

H4Me1K20: Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NBP1-30091B) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NBP1-30091UV
DyLight 405 Labeled NBP1-30091V
DyLight 488 Labeled NBP1-30091G
DyLight 550 Labeled NBP1-30091R
DyLight 650 Labeled NBP1-30091C
DyLight 680 Labeled NBP1-30091FR
DyLight 755 Labeled NBP1-30091IR
FITC Labeled NBP1-30091F
HRP Labeled NBP1-30091H

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Bioinformatics Tool for Histone H4 Antibody (NBP1-30091B)

Discover related pathways, diseases and genes to Histone H4 Antibody (NBP1-30091B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST4H4
Entrez