Histone H4 [Monomethyl Lys20] Antibody (5E10-D8)

Images

 
Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.
Immunocytochemistry/ Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Histone H4 [methyl Lys20] staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) ...read more
Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Hela extracts.
Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Dot blot analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, Dr, Ma, YeSpecies Glossary
Applications WB, ChIP, DB, ELISA, ICC/IF
Clone
5E10-D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) Summary

Immunogen
Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805]
Modification
Monomethyl Lys20
Localization
Nucleus
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HIST4H4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Dot Blot 1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:200
Application Notes
This Histone H4 [Monomethyl Lys20] (5E10-D8) antibody is useful in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence and Western blot. In WB a band is seen at ~11 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-30091 in the following applications:

Reactivity Notes

All mammals including human, mouse and rat, Drosophila, C. elegans, yeast.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8)

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4K20Me1
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

H4Me1K20: Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H4 Antibody (NBP1-30091) (0)

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Review for Histone H4 Antibody (NBP1-30091) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-30091:
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Images Ratings Applications Species Date Details
Western Blot Histone H4 NBP1-30091
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reviewed by:
Anonymous
WB Other 09/12/2016
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Summary

ApplicationWestern Blot
Sample TestedHorse cell lysate
SpeciesOther
FileView PDF

Blocking

Blocking DetailsMilk as blocking agent for 2 hours at 4C

Details

Detection NotesECL

Product General Protocols

View specific protocols for Histone H4 Antibody (NBP1-30091): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NBP1-30091) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-30091AF405
Alexa Fluor 488 Labeled NBP1-30091AF488
Alexa Fluor 647 Labeled NBP1-30091AF647
Alexa Fluor 700 Labeled NBP1-30091AF700
Biotin Labeled NBP1-30091B
DyLight 350 Labeled NBP1-30091UV
DyLight 405 Labeled NBP1-30091V
DyLight 488 Labeled NBP1-30091G
DyLight 550 Labeled NBP1-30091R
DyLight 650 Labeled NBP1-30091C
DyLight 680 Labeled NBP1-30091FR
DyLight 755 Labeled NBP1-30091IR
FITC Labeled NBP1-30091F
HRP Labeled NBP1-30091H

Additional Array Products

Bioinformatics Tool for Histone H4 Antibody (NBP1-30091)

Discover related pathways, diseases and genes to Histone H4 Antibody (NBP1-30091). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Histone H4 Antibody (NBP1-30091)

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PTMs for Histone H4 Antibody (NBP1-30091)

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
09/12/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol HIST4H4
Entrez
Uniprot