Histone H4 [ac Lys5] Antibody (2129F) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10mM Sodium (Na) Butyrate for 24 hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Acetyl Histone H4 (Lys5) Monoclonal Antibody (Catalog # MAB9549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Acetyl Histone H4 (Lys5) at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Histone H4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Acetyl Histone H4 (Lys5) Monoclonal Antibody (Catalog # MAB9549) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 2129F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Histone H4 [ac Lys5] Antibody (2129F) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human Histone H4 synthetic peptide
  Accession # P62805 (H4_Human)
• Modification: ac Lys5
• Specificity: Detects human Acetyl Histone H4 when acetylated at Lys5 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 0.1-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histone H4 [ac Lys5] Antibody (2129F) [Unconjugated]

• H4
• H4/A
• H4/B
• H4/C
• H4/D
• H4/E
• H4/G
• H4/H
• H4/I
• H4/J
• H4/K
• H4/M
• H4/N
• H4/O
• H4/p
• H4F2
• H4FA
• H4FB
• H4FC
• H4FD
• H4FE
• H4FG
• H4FH
• H4FI
• H4FJ
• H4FK
• H4FM
• H4FN
• H4FO
• H4M
• HIST1H4A
• HIST1H4B
• HIST1H4C
• HIST1H4D
• HIST1H4E
• HIST1H4F
• HIST1H4H
• HIST1H4I
• HIST1H4J
• HIST1H4K
• HIST1H4L
• HIST2H4
• HIST2H4A
• HIST2H4B
• HIST4H4
• histone 4, H4
• Histone Cluster 1 H4
• Histone Cluster 1 H4i
• Histone Cluster 4 H4
• histone cluster 4, H4
• Histone H4
• MGC24116

Background

Histone4 (H4/HIST1H4I; also H4M and HIST4H4) is a 12-15 kDa member of the histone H4 family of molecules. Human histone 4 is 103 amino acids (aa) in length. Full length histone 4 is identical in aa sequence to mouse histone 4. H4 is ubiquitously expressed. Histones are the organizing core of nucleosomes, forming an octamer comprised of four histone dimers. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries, mediating transcription regulation, DNA repair, DNA replication and chromosomal stability. Acetylation of the Histone H4 lysine residue at position 5 (K5) reduces the positive charge on the histones, relaxing the nucleosome; the relaxed state is associated with higher levels of gene transcription. Misregulation of acetylation has been linked to developmental and neurodegenerative disorders including Alzheimer's disease, and cancer. Butyryl-CoA has been shown to induce the K5 acetylation on H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P62805 (H4_Human)()