HIF-3 alpha Antibody

Western Blot: HIF-3 alpha Antibody [NB100-2529] - Detection of HIF-3 alpha using NB100-2529. Lane 1: Cos7 (-) control. Lane 2: Cos7 CoCl2 treated (+) control.

Immunocytochemistry/Immunofluorescence: HIF-3 alpha Antibody [NB100-2529] - HIF3 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 549 (red).

Western Blot: HIF-3 alpha Antibody [NB100-2529] - HIF-3a expression in papillary renal cell carcinoma samples. Image from verified customer review.

Product Discontinued

HIF-3 alpha Antibody Summary

• Immunogen: A synthetic peptide made to an internal region of human HIF-3 alpha (within residues 250-350). [Swiss-Prot: Q66K72]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIF3A
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500 - 1:1000
• Application Notes: In Western Blot analysis, a band is recognized at ~68 kDa representing HIF-3 alpha. Because a band at ~71 kDa is also seen in hypoxic samples, it is important to run a negative control lysate as well (see image). In ICC/IF, cytoplasmic and nuclear staining was observed in HeLa cells, which is consistent with the literature under normoxic conditions for HIF-3 alpha (PMID: 16775626).
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for HIF-3 alpha Antibody

• Basic-helix-loop-helix-PAS protein MOP7
• BHLHe17
• bHLHe17HIF-3A2
• Class E basic helix-loop-helix protein 17
• HIF-3 alpha
• HIF3A
• HIF-3A4
• HIF-3-alpha
• HIF3-alpha
• hypoxia inducible factor 3, alpha subunit
• hypoxia-inducible factor 3-alpha
• hypoxia-inducible factor-3 alpha 4
• Inhibitory PAS domain protein
• IPAS
• IPASHIF3-alpha-1
• Member of PAS protein 7
• MOP7
• MOP7PAS domain-containing protein 7
• PASD7
• PASD7HIF-3A

Background

Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIF-3 alpha Antibody (NB100-2529)(7)

Publications using NB100-2529

• Wang L, Cao J, Xu Q et al. 2-Dodecyl-6-Methoxycyclohexa-2,5-Diene-1,4-Dione Ameliorates Diabetic Cognitive Impairment Through Inhibiting Hif3 alpha and Apoptosis Frontiers in Pharmacology 2021-12-16 [PMID: 34975464] (WB, Mouse)
• Tiwari A, Tashiro K, Dixit A Et Al. Loss of HIF1A From Pancreatic Cancer Cells Increases Expression of PPP1R1B and Degradation of p53 to Promote Invasion and Metastasis Gastroenterology 2020-08-03 [PMID: 32768595] (Mouse)
• Heyboer M, Milovanova TN, Wojcik S et al. CD34+/CD45-dim stem cell mobilization by hyperbaric oxygen - Changes with oxygen dosage. Stem Cell Res 2014-03-19 [PMID: 24642336] (FLOW, Human)
• Ando H, Natsume A, Iwami K et al. A hypoxia-inducible factor (HIF)-3alpha splicing variant, HIF-3alpha4 impairs angiogenesis in hypervascular malignant meningiomas with epigenetically silenced HIF-3alpha4. Biochem Biophys Res Commun 2013-02-26 [PMID: 23485455] (WB, Human)
• Storti P, Bolzoni M, Donofrio G et al. Hypoxia-inducible factor (HIF)-1alpha suppression in myeloma cells blocks tumoral growth in vivo inhibiting angiogenesis and bone destruction. Leukemia 2013-01-24 [PMID: 23344526] (WB, Human)
• Forooghian, F et al. Hypoxia-inducible factor expression in human RPE cells. Br J Ophthalmol;91(10):1406-10. 2007-10-01 [PMID: 17567660]
• Wade, KC et al. Gene induction during differentiation of human pulmonary type II cells in vitro. Am J Respir Cell Mol Biol;34(6):727-37. 2006-06-01 [PMID: 16474099]

Review for HIF-3 alpha Antibody (NB100-2529) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pramod Mallikarjuna WB Human 08/30/2014

Summary

• Application: Western Blot
• Sample Tested: papillary renal cell carcinoma samples
• Species: Human

FAQs for HIF-3 alpha Antibody (NB100-2529). (Showing 1 - 1 of 1 FAQs).

1. I'm testing HIF-3A antibody (NB100-2529). I have query about its specificity and expected size: mostly I see band at about 40 kDa instead of 71 (the samples are proteins from cell cultures grown in hypoxic conditions). In your control HIF-3A lysates one H00064344-T02 detecting band at 73 kDa and other H00064344-T01 at 37kDa. Since I'm running the SDS-PAGE, the band at 40kDa might be correct?
   • This protein has multiple isoforms (7) and they might have different expression patterns in various cell lines and tissue. As far as our overexpression control lysates are concerned, H00064344-T02 detects an isoform which is 72kd whereas in H00064344-T01 most probably isoform 4 which is around 40 KD is detected. It is also important to keep in mind that isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secures an inhibition of HIF1A activity. Please see this UniProt link for more information on HIF3A.

Bioinformatics

• Gene Symbol: HIF3A