HGFR/c-MET Antibody (1G7NB) - BSA Free

Immunocytochemistry/Immunofluorescence: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HGFR/c-MET Antibody [1G7NB] (NBP2-44306) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - Analysis of HGF R/c-Met (1G7NB) in human liver cancer.

Flow Cytometry: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - c-MET antibody was tested in HeLa cells (1 x 10^6 cells/ml). After fixation and permeabilization, cells were stained using the anti-c-MET antibody (clone 1G7NB) at a 1:1000 dilution. Signal was detected using a Goat anti-Mouse Dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur. Image using the Azide Free format of this antibody.

Immunocytochemistry/Immunofluorescence: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.

Product Discontinued

HGFR/c-MET Antibody (1G7NB) - BSA Free Summary

• Immunogen: Partial recombinant human c-Met protein (between amino acids 170-400) [UniProt P08581].
• Localization: Transmembrane Protein with Extracellular, Helical and Cytoplasmic Domains
• Predicted Species: Mouse (90%), Rat (90%), Porcine (92%), Bovine (91%), Rabbit (90%), Feline (92%), Canine (92%), Sheep (91%), Primate (100%), Equine (91%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MET
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
• Application Notes: There have been variable results with this antibody in Western blot.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HGFR/c-MET Antibody (1G7NB) - BSA Free

• AUTS9
• cMET
• c-MET
• EC 2.7.10
• EC 2.7.10.1
• hepatocyte growth factor receptor
• HGF R
• HGF receptor
• HGF/SF receptor
• HGFR
• Met (c-Met)
• met proto-oncogene (hepatocyte growth factor receptor)
• met proto-oncogene tyrosine kinase
• MET
• oncogene MET
• Proto-oncogene c-Met
• RCCP2
• Scatter factor receptor
• SF receptor
• Tyrosine-protein kinase Met

Background

The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptorlike protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity.Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines. Tyrosine phosphorylation enhances the receptor kinase activity, while serine phosphorylation of Met on residue 985 has an inhibitory effect. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HGFR/c-MET Antibody (NBP2-44306)(3)

Publications using NBP2-44306

• Karabacak N, Zheng Y, Dubash T, et al. Single cell proteomics of tumor compartments identifies differential kinase activities defining sensitivity to mTOR-PI3-kinase inhibition bioRxiv 2021-01-10 (Mouse)
• Morgan RD, Ferreras C, Peset I et al. c-MET/VEGFR-2 co-localisation impacts on survival following bevacizumab therapy in epithelial ovarian cancer: an exploratory biomarker study of the phase 3 ICON7 trial BMC medicine 2022-02-11 [PMID: 35144591] (IHC-P, Human)
• Karabacak NM, Zheng Y, Dubash TD et al. Differential kinase activity across prostate tumor compartments defines sensitivity to target inhibition Cancer research 2022-01-19 [PMID: 35045985]

Review for HGFR/c-MET Antibody (NBP2-44306) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Cristina Ferreras IF Human 02/12/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Ovarian cancer tissue
• Species: Human

Bioinformatics

• Gene Symbol: MET
• Entrez:
  • 4233(Human)
  • 17295(Mouse)
  • 24553(Rat)
• Uniprot:
  • P08581()
  • P08581-1(Human)