HGF Antibody [Unconjugated]

HGF was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

HGF was detected in perfusion fixed frozen sections of mouse skeletal muscle using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HGF Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse HGF (R&D Systems, Catalog # 2207-HG)
  Gln33-Leu728
  Accession # Q53WS5
• Specificity: Detects mouse HGF in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HGF
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse HGF Antibody (Catalog # AF2207) ELISA Detection: Mouse HGF Biotinylated Antibody (Catalog # BAF2207) Standard:Recombinant Mouse HGF (Catalog # 2207-HG)In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGF Antibody [Unconjugated]

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91%-95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
5. Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
10. Montesano, R. et al. (1991) Cell 67:901.
11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
14. Mineo, R. et al. (1994) Endocrinology 145:4355.
15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HGF Antibody (AF2207)(15)

Publications using AF2207

• Y Deng, Z Zhao, M Sheldon, Y Zhao, H Teng, C Martinez, J Zhang, C Lin, Y Sun, F Yao, H Zhu, L Ma LIFR recruits HGF-producing neutrophils to promote liver injury repair and regeneration bioRxiv : the preprint server for biology, 2023-03-21;0(0):. 2023-03-21 [PMID: 36993315] (Flow Cytometry, ICC, Mouse)
• Eliot Fletcher-Sananikone, Suman Kanji, Nozomi Tomimatsu, Luis Fernando Macedo Di Di Cristofaro, Rahul K. Kollipara, Debabrata Saha et al. Elimination of Radiation-Induced Senescence in the Brain Tumor Microenvironment Attenuates Glioblastoma Recurrence Cancer Research 2021-12-01 [PMID: 34580063]
• Jiayi Yao, Pierre J. Guihard, Xiuju Wu, Ana M. Blazquez-Medela, Melissa J. Spencer, Medet Jumabay et al. Vascular endothelium plays a key role in directing pulmonary epithelial cell differentiation Journal of Cell Biology 2017-10-02 [PMID: 28838957]
• T Ito, D Yamaji, A Kamikawa, MA Abd Eldaim, Y Okamatsu-O, A Terao, M Saito, K Kimura Progesterone dose-dependently modulates hepatocyte growth factor production in 3T3-L1 mouse preadipocytes Endocr. J., 2017-07-27;0(0):. 2017-07-27 [PMID: 28659539] (Western Blot, Mouse)
• P Bendinelli, P Maroni, E Matteucci, MA Desiderio Epigenetic regulation of HGF/Met receptor axis is critical for the outgrowth of bone metastasis from breast carcinoma Cell Death Dis, 2017-02-02;8(2):e2578. 2017-02-02 [PMID: 28151481] (IHC, Mouse)
• Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma PLoS ONE, 2016-05-25;11(5):e0156151. 2016-05-25 [PMID: 27223472] (Mouse)
• Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M MET is required for the recruitment of anti-tumoural neutrophils. Nature, 2015-05-18;522(7556):349-53. 2015-05-18 [PMID: 25985180] (Neutralization, Human, Mouse)
• Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death. Cell Death Differ, 2015-01-09;22(7):1219-30. 2015-01-09 [PMID: 25571972] (IHC, Neutralization, Mouse)
• Wu H, Yiu W, Li R, Wong D, Leung J, Chan L, Zhang Y, Lian Q, Lin M, Tse H, Lai K, Tang S Mesenchymal stem cells modulate albumin-induced renal tubular inflammation and fibrosis. PLoS ONE, 2014-03-19;9(3):e90883. 2014-03-19 [PMID: 24646687] (Neutralization, Mouse)
• Kishore , Raj, Verma , Suresh K, Mackie , Alexande, Vaughan , Erin E, Abramova , Tatiana, Aiko , Ito, Krishnamurthy , Prasanna Bone marrow progenitor cell therapy-mediated paracrine regulation of cardiac miRNA-155 modulates fibrotic response in diabetic hearts. PLoS ONE, 2013-04-01;8(4):e60161. 2013-04-01 [PMID: 23560074] (Neutralization, Mouse)
• Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis. Cancer Res, 2013-02-27;73(8):2659-70. 2013-02-27 [PMID: 23447577] (Western Blot, Mouse)
• Gordin M, Tesio M, Cohen S c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74. J. Immunol., 2010-07-16;185(4):2020-31. 2010-07-16 [PMID: 20639480] (Flow Cytometry, Neutralization, Mouse)
• Tesio M, Golan K, Corso S Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling. Blood, 2010-06-28;117(2):419-28. 2010-06-28 [PMID: 20585044] (ELISA Development, Flow Cytometry, Mouse)
• Narasaraju T, Ng HH, Phoon MC MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis. Am. J. Respir. Cell Mol. Biol., 2009-07-17;42(6):732-43. 2009-07-17 [PMID: 19617401] (IHC-P, Mouse)
• Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells. Am. J. Pathol., 2006-11-01;169(5):1767-83. 2006-11-01 [PMID: 17071599] (IHC, Mouse)

Reviews for HGF Antibody (AF2207) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 01/06/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23074208
• Species: Mouse

reviewed by: Verified Customer IHC-P Mouse 01/06/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 24218051
• Species: Mouse

Bioinformatics

• Gene Symbol: HGF
• Uniprot:
  • Q53WS5()