HEY1 Antibody

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Western Blot: HEY1 Antibody [NBP2-27152] - Analysis of Hey1 in mouse embryo brain lysate using 1) preimmune sera at 1:5000 dilution and 2) purified NBP2-27152. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HEY1 Antibody Summary

Immunogen
A portion of amino acids 50-100 of mouse Hey1 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HEY1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 2-4ug/ml
Application Notes
This antibody has been tested in Western blot on mouse tissue. The Western blot results on human tissue have been mixed.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for HEY1 Antibody

  • basic helix-loop-helix protein OAF1
  • BHLHb31
  • Cardiovascular helix-loop-helix factor 2
  • CHF-2
  • CHF2hHRT1
  • Class B basic helix-loop-helix protein 31
  • Hairy and enhancer of split-related protein 1
  • hairy/enhancer-of-split related with YRPW motif 1
  • hairy/enhancer-of-split related with YRPW motif protein 1
  • Hairy-related transcription factor 1
  • HERP2HES-related repressor protein 1
  • HESR-1
  • HESR1MGC1274
  • HES-related repressor protein 2
  • HRT1
  • HRT-1OAF1

Background

Hairy/enhancer-of-split related with YRPW motif protein 1 belongs to the HEY family and is a Notch Signaling Pathway Protein. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. It is a downstream effector of Notch signaling which may be required for cardiovascular development. It acts as transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. It represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Hey1 is also reported to be expressed during development of the nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HEY1
Uniprot