HEY1 Antibody

Images

 
Western Blot: HEY1 Antibody [NBP2-16818] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody diluted at 1:5000. The ...read more
Immunocytochemistry/ Immunofluorescence: HEY1 Antibody [NBP2-16818] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HEY1 protein stained by HEY1 antibody diluted at 1:2000. Red: alpha Tubulin, a ...read more
Immunohistochemistry-Frozen: HEY1 Antibody [NBP2-16818] - Hey1 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody dilution at 1:100. Secondary antibody at 1:1000, ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HEY1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HEY1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (96%), Bovine (93%), Canine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HEY1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:1000 - 1:10000
Application Notes
HEY1 Antibody is validated for IHC-Fr from a verified customer review.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-16818 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%), Xenopus laevis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for HEY1 Antibody

  • basic helix-loop-helix protein OAF1
  • BHLHb31
  • Cardiovascular helix-loop-helix factor 2
  • CHF-2
  • CHF2hHRT1
  • Class B basic helix-loop-helix protein 31
  • Hairy and enhancer of split-related protein 1
  • hairy/enhancer-of-split related with YRPW motif 1
  • hairy/enhancer-of-split related with YRPW motif protein 1
  • Hairy-related transcription factor 1
  • HERP2HES-related repressor protein 1
  • HESR-1
  • HESR1MGC1274
  • HES-related repressor protein 2
  • HRT1
  • HRT-1OAF1

Background

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HEY1 Antibody (NBP2-16818) (0)

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Review for HEY1 Antibody (NBP2-16818) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-16818:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen HEY1 NBP2-16818
Enlarge
4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/07/2021
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedE11.5 mouse embryo fixed in 4% PFA
SpeciesMouse
Lot43096

Comments

CommentsFixed 4% PFA overnight.
Blocked with 1% BSA
Primary antibody dilution - 1:100
Secondary antibody - Invitrogen Alexa Fluor 488
Secondary antibody dilution - 1:1000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HEY1 Antibody (NBP2-16818) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

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Recent Reviews

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4
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1
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Verified Customer
01/07/2021
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol HEY1
Uniprot