Rat Hemopexin ELISA Kit (Chemiluminescence)

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ELISA: Rat Hemopexin ELISA Kit (Chemiluminescence) [NBP2-68027] - Samples were spiked with high concentrations of Rat Hemopexin and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat Hemopexin ELISA Kit (Chemiluminescence) [NBP2-68027] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

Rat Hemopexin ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Rat HPX in samples. No significant cross-reactivity or interference between Rat HPX and analogues was observed.
Standard Curve Range
15.63-1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 11.1%
Intra-Assay
CV% < 8.85%
Spike Recovery
93-115%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
HPX

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Rat Hemopexin ELISA Kit (Chemiluminescence)

  • Beta-1B-glycoprotein
  • FLJ56652
  • Hemopexin
  • HPX
  • HX

Background

The Hemopexin gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phaseprotein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidativestress. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Hemopexin ELISA Kit (NBP2-68027). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol HPX